A lectin histochemical study of the zygomatic salivary gland of adult dogs |
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Authors: | V Pedini P Ceccarelli A M Gargiulo |
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Institution: | (1) Istituto di Anatomia degli Animali Domestici, Facoltà di Medicina Veterinaria, Via S. Costanzo 4, 06126 Perugia, Italy |
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Abstract: | Seven lectins (PNA, DBA, SBA, UEA I, LTA, WGA and ConA), conjugated with horseradish peroxidase, were used to characterize the glycosidic residues in the zygomatic gland of adult dogs. In some cases (PNA and DBA), lectin staining was preceded by neuraminidase digestion. The acinar and tubular cells produced glycoconjugates with different sugar residues, presenting binding sites for all of the lectins used. The apical surfaces of the cells lining the intra- and interlobular ducts were also stained by all the lectins. In contrast, the demilunar cells only reacted with the Neu-PNA sequence and Con A.
Abbreviations: Neu, neuraminidase; see also Table I |
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Keywords: | dog glycoconjugate histochemistry lectin salivary gland zygomatic |
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