云芝子实体氯仿提取物体外抗肿瘤活性研究

研究了云芝子实体氯仿提取物（CVFC）对人肝癌细胞株HepG2的体外抑制作用，并初步探讨了抑瘤机制。MTr结果显示，CVFC对HepG2细胞有明显的抑制作用，并呈明显的量效关系，半数抑制浓度为（75．83±3．96）μg·mL^-1。荧光显微镜和透射电镜形态观察发现CVFC处理可使细胞发生细胞皱缩、核碎裂等凋亡特征；Annexin V/PI双染结果证实有胞膜磷脂酰丝氨酸的翻转，经150μg·mL^-1 CVFC处理HepG2细胞24h后早期凋亡率为17．82％，晚期凋亡率为22．59％，均显著高于对照组5．35％和3．75％。这些结果表明CVFC具有体外抑制HepG2细胞的活性，其抑瘤机制与诱导肿瘤细胞凋亡有关。

In Vitro Anti-tumor Effects of Chloroform Extract from Trametes versicolor Fruiting Body

In this report, in vitro the inhibition and cytotoxic effects of a chloroform extract from Trametes versicolor fruiting body （CVFC） was studied using a liver carcinoma HepG2 cell line. MTF assay results showed the CVFC inhibited HepG2 cells growth in a dose-dependent manner. The IC50 was found as （75.83±3.96） μg·mL^-1. Cell morphological observation using fluorescent microscope and transmission electron microscope revealed that the CVFC treated cells presented typical apoptotic characteristics, including cell shrinkage, nuclear condensation and fragmentation. Annexin V/PI staining assay confirmed that when HepG2 cells treated with CVFC at a concentration of 150 μg·mL^-1 for 24 h, the cell membrane phosphatidylserine flipped out, which was a golden mark of apoptosis. The percentage of early and late apoptosis cells were 17.82% and 22.59% respectively, much higher than that of the control （5.35% and 3.75% respectively）. These results suggested that CVFC had an inhibition effect on HepG2 in vitro and mechanism may be through inducing apoptosis.