基于SSR标记的食荚菜豆指纹图谱构建

以14个食荚菜豆品种为试材,采用SSR分子标记技术,对其进行遗传多样性研究,并建立了特异性指纹图谱,以期为今后食荚菜豆的品种鉴定建立新的参考依据,同时也为菜豆种质资源的分类和筛选提供分子生物学的参考方法。结果表明:74对SSR引物中有18对引物在供试样品中表现出多态性,筛选出其中5对具有良好多态性SSR引物(BM200、PVBR269、PVBR6、BM141、BM154)用于构建指纹图谱,并对14个食荚菜豆品种进行聚类分析。在相似系数为0.64时,14个食荚菜豆品种被分为2个类群,聚类结果显示"黑珍珠"和"黄金钩"的相似度最高,相似系数为0.98。最终构建菜豆指纹图谱,对深入挖掘菜豆种质资源,减少亲本选配的盲目性,提高育种工作效率,并对品种鉴定和知识产权保护均具有重要的应用意义。

Construction of Fingerprint of Viburnum Bean Based on SSR Marker

The 14 bean cultivars were used as materials, 14 SSR molecular markers were used to study the genetic diversity and specific fingerprints were established,in order to establish a new reference for the identification of snap beans in the future,it also provided a reference method of molecular biology for identifying and classifying the germplasm resources of the snap beans. The results showed that 18 of 74 pairs of SSR primers showed polymorphisms in snap bean samples,and 5 SSR primers(BM200, PVBR269,PVBR6, BM141, BM154) were selected for fingerprint establishment and genetic cluster analysis of 14 bean varieties. The results of clustering showed that 14 snap bean cultivars were divided into two groups at the similarity coefficient of 0.64. The clustering results also suggested that the genetic distance both of ‘Heizhenzhu' and ‘Huangjingou' was the closest,with a similarity coefficient of 0.98.To construct the fingerprint of the snap beans had a great useful value for identifying the germplasm resources of the snap beans, the selection of parent, increasing the efficiency of breeding and intellectual property protection.