报春石斛组培快繁技术体系的建立

以报春石斛蒴果为外植体,通过对种子无菌萌发和原球茎诱导、原球茎增殖及丛生芽诱导、生根壮苗以及移栽驯化的研究,以期建立报春石斛的组培快繁技术体系。结果表明:培养基MS+6-BA 1.0mg·L^-1+NAA 0.1mg·L^-1+蔗糖20g·L^-1最适报春石斛种子无菌萌发和原球茎的诱导;原球茎增殖及丛生芽诱导的最佳培养基为MS+6-BA1.0mg·L^-1+NAA 0.1mg·L^-1+香蕉泥100g·L^-1+蔗糖20g·L^-1,继代培养45d,增殖系数达8.2;最佳生根壮苗培养基为MS+6-BA 1.0mg·L^-1+KT 0.5mg·L^-1+NAA 0.2mg·L^-1+香蕉泥100g·L^-1+蔗糖30g·L^-1+活性碳1.0g·L^-1,培养3个月,生根率达100%,根系较长,苗壮;3—4月是桂林地区移栽报春石斛组培苗的最佳季节,生根苗在栽培基质Ⅰ(树皮∶水苔∶珍珠岩∶蛭石=10∶2∶1∶1,体积比)的长势好于栽培基质Ⅱ(树皮∶珍珠岩∶蛭石=10∶1∶1,体积比),成活率为92.3%。

Establishment of Tissue Culture and Rapid Propagation System of Dendrobium primulinum

In order to establish a rapid propagation system of D.primulinum,immature seeds of Dendrobium primulinum Lindl.were used as explants for tissue culture,and mediums for aseptic seed germination,subculture of protocom,rooting and growth of strong plantlets were screened.The results showed that the medium MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+sugar 20g·L^-1 was optimum for seed germination.The optimum subculture medium that for proliferation of protocorm and shoot induction was MS+6-BA 1.0mg·L^-1+NAA 0.1mg·L^-1+mashed banana 100g·L^-1+sugar 20g·L^-1,the propagation coefficient was 8.2after subcultured 45 days.The optimum rooting medium was MS+6-BA 1.0 mg·L^-1+KT 0.5 mg·L^-1+NAA 0.2 mg·L^-1+mashed banana100g·L^-1+sugar 30g·L^-1+activated carbon 1.0g·L^-1,the rooting rate reached 100% and the roots had higher quality and more quantity after cultured 90 days.The best time for the transplantation of root regeneration plants was March to April in Guilin area,the rooted plants grew better in mediaⅠ(pine bark∶sphagna∶perlite∶vermiculite=10∶2∶1∶1)than that in mediaⅡ(pine bark∶perlite∶vermiculite=10∶1∶1),and the survival rate was 92.3%.