MoFbc1调控稻瘟病菌生长与致病机制初探

真核生物体内,F-box蛋白作为泛素连接酶复合物(Skp1-Cullin1-F-box,SCF)的调节亚基,参与降解底物的特异性识别。为探究F-box家族基因MoFbc1在稻瘟病菌生长发育及侵染致病中的功能,采用生物信息学方法分析Mo Fbc1蛋白结构域并构建进化树。利用同源重组方法获得MoFbc1基因敲除及其回补菌株并进行表型分析。结果表明,MoFbc1敲除菌株在产孢、附着胞形成及致病性等方面与野生型菌株均无显著差异。但其在MM和RDC培养基上生长速率下降,表明MoFbc1基因可能参与调控稻瘟病菌对部分营养物质的利用;突变体对细胞壁胁迫因子CFW、CR敏感,提示其细胞壁结构可能发生改变。结果为进一步揭示基因MoFbc1调控稻瘟病菌生长发育机制奠定基础。

Identification of MoFbc1 during the development and pathogenicity in Magnaporthe oryzae

In eukaryotes, F-box proteins are regulatory subunits of the ubiquitin ligase complex (Skp1- Cullin1-F-box, SCF) and involved in the specific recognition of degraded substrates. To explore the function of the F-box family gene MoFbc1 in M. oryzae, the protein domain of MoFbc1 was analyzed by bioinformatics method and the phylogenetic tree was constructed. Homologous recombination techniques were used to obtain the knockout and complementary strains of MoFbc1 gene. The knockout strains exhibited no significant difference to wild-type in conidiation, appressorium formation and virulence. However, the decrease in the growth rate on MM and RDC cultures indicated that the mutant of MoFbc1 gene may have abnormal utilization of some nutrients. It was sensitive to the cell wall stressors CFW and CR, suggesting that alteration of cell wall integrity of MoFbc1 may occur in the MoFbc1 mutants. The results would provide basis for further revealing the regulatory mechanism of MoFbc1 in the growth and development of M. oryzae.