禾谷镰刀菌脂质转运蛋白Vps13的功能分析

 Vps13蛋白家族是真核生物中高度保守的一类脂质转运蛋白,其在丝状真菌中的功能尚不清楚。小麦赤霉病主要由禾谷镰刀菌(Fusarium graminearum)引起,是小麦最重要病害之一。禾谷镰刀菌含有酿酒酵母VPS13的一个同源基因FgVPS13。通过同源重组的方法获得禾谷镰刀菌FgVPS13敲除突变体。研究表明,FgVPS13敲除突变体出现生长、产孢和有性生殖的缺陷。FgVPS13敲除突变体在小麦胚芽鞘和麦穗上的致病力下降,产生的脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)毒素含量也明显降低。而且,FgVPS13抑制线粒体自噬。总之,FgVPS13参与调控禾谷镰刀菌菌丝生长、无性和有性生殖、致病力和线粒体自噬。

Functional analysis of the lipid transfer protein Vps13 in Fusarium graminearum

The Vps13 (vacuolar protein sorting 13) lipid transfer family is highly conserved in eukaryotic cells, the role of Vps13 in filamentous fungi remains unclear. Fusarium head blight caused by Fusarium graminearum is one of the most important diseases of wheat. A single FgVPS13 gene, orthologous to the yeast VPS13, was identified in F. graminearum. FgVPS13 deletion mutants were acquired through homologous recombination in this study. Results showed that the FgVPS13 deletion mutant was defective in mycelial growth, conidiation, and sexual reproduction in F. graminearum. The pathogenicity of the FgVPS13 mutant was significantly reduced in wheat coleoptiles and flowering wheat heads, DON production was also reduced compared with the wild type PH-1. In addition, FgVPS13 suppressed mitophagy in F. graminearum. In summary, these results suggest that FgVPS13 regulates mycelial growth, conidiation, sexual reproduction, pathogenicity, and mitophagy in F. graminearum.