白僵菌生产工艺规范化技术的研究

通过对白僵菌不同工艺技术生产菌粉产孢量比较研究 ,筛选出工艺规范化生产各环节的最优组合技术参数 ,提出二级液体 (7%麦皮煮出液 )和三级固体培养基 (麦皮∶谷壳 =9∶1)及其液固接种量 (1∶1)最适配方 ,并确定前 3d三级固体开放培养发酵最适温度为 18～ 2 2℃、相对湿度为 95 %～ 10 0 % ,还总结出以二级液体振荡培养 6 0h接种 ,三级固体发酵培养 7～10d ,可获得最优质的菌粉 ,平均含孢量达 2 0 0× 10 8个·g-1以上 ,缩短生产周期 1/3 ,降低生产成本 ,增加菌厂经济收益 ,为菌厂大量生产提供参数依据。

Study on the Standardized Techniques of Beauveria bassiana Production Technology

The optimal combination technique parameters of various links in the technological standardization production were selected through the comparative study.The second grade liquid media with 7% of bran decoction,the third grade solid media with the proportion of bran:chaff=9∶1,and the optimum formulation of their inoculum concentration (1∶1) were put forward.The best temperature (18～22℃) and relative humidity (95%～100%)were determined for the third-grade solid open culture fermentation for the first three days.It was also summarized that the optimal quality Beauveria bassiana powder with 200×10 8 spores per g could be obtained by shakily culturing the second grade liquid media for 60 hours and then inoculating it and by fermentatively culturing the third grade solid media for 7～ 10 days,the productive cycle was shortened by 1/3,the productive cost was reduced,the economic benefit of B. bassiana was increased,and the parameter basis was provided for the mass production in the factory.