四个马铃薯品种幼茎段再生技术的研究

以4个马铃薯栽培品种为试材,进行了其茎段的离体再生研究。结果表明,东农303、夏波帝、鄂1 号茎段愈伤组织最佳诱导培养基为MS+6-BA 3．0 mg／L+NAA 0．01 mg／L;费乌瑞它为MS+6-BA 3．0 mg／L+ NAA 0．1mg／L,愈伤组织的诱导率均可达100％。费乌瑞它和鄂1号不定芽分化诱导的最佳培养基均为MS+ 6-BA 2．0 mg／L+NAA 0．2 mg／L;东农303为MS+6-BA 2．0 mg／L+NAA 0．1 mg／L:夏波帝为MS+6-BA 2．0 mg／L+NAA 0．3 mg／L,其不定芽分化率分别达96．7％．96．7％,83．3％和80．0％。各品种不定芽的诱导生根率均为100％,试管苗移栽成活率为95％。

Study on in vitro regeneration technology from stem explants of four potato cultivars

Four potato cultivars were regenerated in vitro from stem explants. The results showed that the optimal medium for callus induction was found to be MS + 6-BA 3. 0 mg/L + NAA 0. 01 mg/L for cv. Dongnong 303,cv. Shepody and cv. E No. 1, MS + 6-BA 3. 0 mg/L + NAA 0. 1 mg/L for cv. Favorite. The callus induction rate on those media reached up to 100%. The optimal medium for adventitious bud differentiation from stem callus was found to he MS+6-BA 2. 0 mg/L + NAA 0. 2 mg/L for cv. Favorite and cv. E No. 1,MS+6-BA 2. 0 mg/L + NAA 0. 1 mg/L for cv. Dongnong 303,and MS + 6-BA 2. 0 mg/L + NAA 0. 3 mg/L for cv. Shepody. The adventitious hud differentiation rate reached 96. 7%, 96. 7%, 83. 3% and 80. 0% for the four cultivars respectively,and the rooting rate was 100% for all cultivars. The survival rate of tube plantlets after transplantation reached 95%.