Nicotinic Acetylcholine Receptor Gene Family of the Pea Aphid,Acyrthosiphon pisum |
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Authors: | LIU Yi-peng LIN Ke-jian LIU Yang GUI Fu-rong WANG Gui-rong |
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Institution: | [1]State Key Laboratory for Biology of Plant Diseases and lnsect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P,R.China [2]College of Plant Protection, Yunnan Agricultural University, Yunnan 650201, P.R.China |
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Abstract: | The nicotinic acetylcholine receptors (nAchRs) are cholinergic receptors that form ligand-gated ion channels by five subunits in insect and vertebrate nervous systems. The insect nAChR is the molecular target of a class of insecticides, neonicotinoids. Here, we identified and cloned 11 candidate nAChR subunit genes in Acyrthosiphon pisum using genome-based bioinformatics combined modern molecular techniques. Most A. pisum nAChRs including α1, α2, α3, α4, α6, α8, and β1 show highly sequence identities with the counterparts of other insects examined. Expression profiles analysis showed that all subunit genes were expressed in adult head. At least two subunits have alternative splicing that obviously increase A. pisum nicotinic receptor diversity. This study will be invaluable for exploring the molecular mechanisms of neonicotinoid-like insecticides in sucking pests, and for ultimately establishing the screening platform of novel insecticides. |
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Keywords: | Acyrthosiphon pisum nicotinic acetylcholine receptor alternative splicing expression profile |
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