| 海口地区产脂肪酶菌株的分离与16S rRNA序列分析 |
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| 引用本文： | 潘晓磊,廖承红,丛茜,李晓陀,郭桂英,张旭,迟亭亭,郑继平.海口地区产脂肪酶菌株的分离与16S rRNA序列分析[J].热带生物学报,2012,3(1):28-31. |
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| 作者姓名： | 潘晓磊 廖承红 丛茜 李晓陀 郭桂英 张旭 迟亭亭 郑继平 |
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| 作者单位： | [1]海南省热带生物资源可持续利用重点实验室／省部共建国家重点实验培育基地,海南海口570228 [2]海南大学热带生物资源教育部重点实验室 ,海南海口570228 [3]海南大学教务处,海南海口570228 [4]海南大学农学院,海南海口570228 |
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| 基金项目： | 教育部科学技术研究重点项目（208111）;海南大学青年基金（qnjj1147） |
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| 摘 要： | 以橄榄油为碳源，采用富集和复筛的方法，于夏季从海口市食堂和汽修场等富油土壤中筛选出9株高产脂肪酶活性菌株，对其形态、生长和16SrRNA序列进行分析，结果表明：各菌株菌落皆呈暗绿色，镜检为革兰氏阴性、杆状单细胞，最适生长pH值为7，最适生长温度高于42℃，9株菌株的16SrDNA序列完全一致，皆为绿脓假单胞菌。
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| 关 键 词： | 脂肪酶 16S rRNA 绿脓假单胞菌 海口 |
Isolation and 16S rRNA gene analysis of lipase strains in Haikou in Hot Summer |
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| Institution: | PAN Xiao-lei,LIAO Cheng-hong, CONG Qian, LI Xiao-tuo, GUO Gui-ying, ZHANG Xu, CHI Ting-ting , ZHENG jl-ping ( 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-resources-State Key Laboratory Incubation Base ; 2. Ministry of Education Key Laboratory forTropical Biological Resources, Hainan University, Haikou 570228, China; 3. Academic Affairs Office, Hainan University, Haikou 570228, China; 4. College of AgronOmy, Hainan University, Haikou 570228, China) |
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| Abstract: | Nine high producing lipase strains were isolated from rich oil soil at canteens, sea sludge and garages from Haikou in hot summer. All these strains were Gram-negative and rod-shaped single cells and investigated in terms of morphology, growth and 16S rRNA gene sequences. All colonies of these strains were blue green on the plate under light microscope, indicating that the strains might secrete pigments of such color. The optimal growth conditions for these strains were pHT. 0 at the temperature of above 42℃. All the strains had completely the same 16S rRNA gene sequences as Pseudomonas aeruginosa, and hence are identified as P. aeruginosa. |
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| Keywords: | lipase 16S rRNA Pseudomonas aeruginosa Haikou |
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