初始接种量对安徽虫瘟霉液体培养下菌丝生物量的影响

在温度（２０±１）℃和光照１２Ｌ ： １２Ｄ的条件下，用萨氏培养液对安徽虫瘟霉（Ｚｏｏｐｈｏｒａ　ａｎ－ ｈｕｉｅｎｓｉｓ）进行液体振荡培养（１００ ｒ／ｍｉｎ），考查不同接种量和液培时间对菌丝生物量的影响。结果表明， 将含干菌丝１８． ６ｍｇ／ｍＬ的母液按１％～１５％（Ｖ／Ｖ）的比例接种后培养４８～７２ｈ，９％和１２％接种量处 理能产生２０ ｍｇ／ｍＬ 以上的菌丝生物量，通过拟合逻辑斯蒂生长模型，发现该菌在上述液培条件下的最 大菌丝生物量为２５ ｍｇ／ｍＬ左右。

The amount of initial inocula affects the mycelial biomass of the entomophthoralean fungus(Zoophthora anhuiensis) in liquid culture.

The entomophthoralean fungus, Zoophthora anhuiensis,was investigated in shaking (100 r/min) culture of Sabouraud dextrose broth at the regime of 20 and 12L : 12D to evaluate the effect of initial inocula on mycelial biomass. Seed culture containing 18. 6 mg dry mycelia per milliliter was used to initiate 50 mL liquid culture at the proportions (V/V) of 1%/~15%. It was found that after incubation of 48~72 h the cultures initiated 9% and 12% seed culture yielded a mycelial biomass of >20 mg/mL. The firting of the data to Logistic growth medel showed that Z. anhuiensis may produce a maximum biomass of ca. 25mg/mL at the above regime.