| 芸薹属DFR基因家族RNA干扰载体的构建 |
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| 引用本文： | 呼丽君,柴友荣,张建奎,姚永宏.芸薹属DFR基因家族RNA干扰载体的构建[J].西南农业学报,2011,24(3). |
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| 作者姓名： | 呼丽君 柴友荣 张建奎 姚永宏 |
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| 作者单位： | 1. 西南大学农学与生物科技学院，农业部生物技术和作物品质改良重点实验室，重庆市油菜工程中心，重庆市作物品质改良重点实验室，重庆，400716
2. 重庆市农业科学院，重庆，401329 |
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| 基金项目： | 国家“863”计划项目(2006AA10Z110、2006AA10A113); 国家自然科学基金(30771237); 重庆市科技攻关项目(CSTC2009AB1030) |
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| 摘 要： | 二氢黄酮醇-4-还原酶(dihydroflavonol-4-reductase,DFR)是类黄酮途径的一个关键酶,对种皮色泽和花色的形成具有重要作用。本研究从甘蓝型油菜克隆了600 bp(不计人工酶切位点)的芸薹属DFR基因家族的RNA干扰片段BDFRI,将其反义片段和正义片段分别插入到本课题组新近改造的植物RNAi平台载体pFGC5941M的启动子与间隔区之间、间隔区与终止子之间,构建了11 400 bp的RNAi干扰载体pFGC5941M-BDFRI,通过多重PCR鉴定证实载体构建成功,并转化到根癌农杆菌菌株LBA4404中形成了工程菌株。此载体的构建为进一步研究DFR基因参与决定芸薹属种皮色素和茎叶彩色性状的分子机理和代谢调控奠定了基础。
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| 关 键 词： | 芸薹属 甘蓝型油菜 二氢黄酮醇-4-还原酶(DFR) RNA干扰 种皮颜色 载体构建 |
Vector Construction for RNAi of Brassica DFR Gene Family |
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| HU Li-jun,CHAI You-rong,ZHANG Jian-kui,YAO Yong-hong.Vector Construction for RNAi of Brassica DFR Gene Family[J].Southwest China Journal of Agricultural Sciences,2011,24(3). |
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| Authors: | HU Li-jun CHAI You-rong ZHANG Jian-kui YAO Yong-hong |
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| Institution: | HU Li-jun1,CHAI You-rong1,ZHANG Jian-kui1,YAO Yong-hong2(1.Chongqing Key Laboratory of Crop Quality Improvement,Chongqing Rapeseed Engineer Research Centre,Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture,College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China,2.Chongqing Academy of Agricultural Sciences,Chongqing 401329,China) |
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| Abstract: | Dihydroflavonol-4-reductase(DFR) is a key enzyme of phenylpropanoid-flavonoid pathway.It plays important roles in pigmentation of seed coat and plant surface.A 600-bp(not including the artificial enzyme sites) RNAi fragment,BDFRI,targeted to Brassica DFR gene family was cloned from Brassica napus.Its antisense and sense fragments were inserted to the promoter-spacer and spacer-terminator cloning sites of plant RNAi platform vector pFGC5941M which was modified by this research team recently,forming the 11400... |
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| Keywords: | Brassica Brassica napus Dihydroflavonol-4-reductase(DFR) RNA interference Seed color Vector construction |
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