人鼻咽上皮裸小鼠移植及诱癌实验中的EB病毒DNA检测

人鼻咽组织移植于２０只裸小鼠，分三组再分别多次以ＥＢＶ（７头）、ＥＢＶ＋ＴＰＡ（５头）或生理盐水（对照８头）皮下注射，诱癌后处死，将移植物作常规病理切片观察，并同时提取ＤＮＡ为模板，以ＥＢ病毒ＢａｍＨＩＷ片段特异性引物作ＰＣＲ检测ＥＢ病毒。结果显示：ＥＢＶ组与ＥＢＶ＋ＴＰＡ组中，７只可见鼻咽组织移植物发生癌前或癌变（原位癌、早期浸润癌各１只），而对照组均未见上述改变，两组比较差别有显著意义（０．０１＜Ｐ＜０．０５）；无形态变化的受试动物中ＩＢ病毒阳性２只，阴性１１只；有癌前病变和癌变的动物中，阳性５只，阴性２只，两组差别有显著性（０．０１＜Ｐ＜０．０５），提示观察到的病变与ＥＨ病毒感染有关。

THE EB VIRUS DETECTION BY PCR IN EXPERIMENTAL CARCINOGENESIS OF HUMAN NASOPHARYNGEAL EPITHELIA TRANSPLANTED INTO NUDE MICE

The nude mice with transplants of human nasopharyngeal epithelia(NE)were repeatedly injected subdermally by EBV, EBV+TPA or distillted water, respectively.Transplants were sectioned finally and HE stained, DNAs were extracted for PCRs using the primers specific for EBV DNA BamHI W fragment.The results showed that EBV or EBV+TPA induced not only precancerious changes(5/12),but also carcinomas(2/12)of transplanted NE, they were not found in the control animals.Of 20 cases,2 were positive and 11 negative of EBV PCR in non-change group,however,5 positive and 2 negative in precancerious and cancerious group.There were significant differences between these two groups(0.01