| 稻瘟病菌温度敏感型突变体的筛选 |
| |
| 引用本文： | 王洪凯,林福呈,柴荣耀,李德葆.稻瘟病菌温度敏感型突变体的筛选[J].中国水稻科学,2004,18(4):357-361. |
| |
| 作者姓名： | 王洪凯 林福呈 柴荣耀 李德葆 |
| |
| 作者单位： | 1浙江大学 生物技术研究所, 浙江 杭州 310029； 2浙江大学 生命科学学院, 浙江 杭州 310029； 3浙江省农业科学院 植物保护与微生物研究所, 浙江 杭州 310021 |
| |
| 基金项目： | 国家自然科学基金资助项目 ( 3 0 0 80 0 2 1) |
| |
| 摘 要： | 通过紫外诱变，筛选获得了274个稻瘟病菌的温度敏感型突变体，这些突变体在25℃下可以生长，而在33℃下不能生长。致病性测定结果表明，有32个突变体的致病性显著降低; 在33℃条件下，不萌发的有7个；有12个萌发后停止生长，不能形成附着胞；有9个可以形成附着胞，以后停止生长；有4个形成膨大透明且成串生长的附着胞，随后停止生长。有些温度敏感型突变体形成子囊壳的能力也发生了改变。
|
| 关 键 词： | 稻瘟病菌 温度敏感型突变体 萌发 附着胞 子囊壳 |
| 文章编号： | 1001-7216(2004)04-0357-05 |
| 收稿时间： | 1900-01-01; |
Screening of Temperature Sensitive Mutants in Magnaporthe grisea |
| |
| WANG Hong kai ,LIN Fu cheng ,CHAI Rong yao ,LI De bao.Screening of Temperature Sensitive Mutants in Magnaporthe grisea[J].Chinese Journal of Rice Science,2004,18(4):357-361. |
| |
| Authors: | WANG Hong kai LIN Fu cheng CHAI Rong yao LI De bao |
| |
| Institution: | （ ;1. Biotechnology Institute; Zhejiang University; Hangzhou 310029; China;2. College of Life Sciences;3. Institute of Plant Protection and Microbiology; Zhejiang Academy of Agricultural Sciences; Hangzhou 310021; China） |
| |
| Abstract: | Temperature sensitive mutants(ts mutants) are good material for studying cell division and differentiation. Two hundred and seventy four ts mutants of Magnaporthe grisea were induced by UV radiation. These mutants could grow at 25℃, but not at 33℃. Pathogenicity test indicated that the pathogenicity of 32 ts mutants reduced distinctly. Under 33℃ conditions, seven of them did not germinate, 12 stopped growing after germination and no appressorium formed, nine stopped growing after appressorium formed, four mutants formed swell and bunch appressorium, then stopped growing. The ability of perithecia formation of some mutants was changed. |
| |
| Keywords: | Magnaporthe grisea temperature sensitive mutant germination appressorium perithecia |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国水稻科学》浏览原始摘要信息 |
| 点击此处可从《中国水稻科学》下载免费的PDF全文 |
