苎麻悬浮细胞原生质体培养再生植株

苎麻品种浏阳大叶绿的子叶在含有２，４－Ｄ０．５ｍｇ／Ｌ、ＫＴ０．５ｍｇ／Ｌ的ＭＳＢ固体培养基上，形成愈伤组织。愈伤组织经３￣４次继代培养后作液体振荡培养，产生悬浮细胞系。从悬浮系分离的原生质体，只有以海藻酸钠包埋方式培养在ＫＭ８Ｐ培养基中，５０天左右才能形成肉眼可见的小愈伤组织。该愈伤组织在附加２，４－Ｄ０．２ｍｇ／Ｌ、６－ＢＡ０．１ｍｇ／Ｌ的ＭＳＢ生长培养基上增殖，然后转入附加６－ＢＡ２．０ｍｇ

Plant Regeneration from Protoplasts of Suspension Cells of Ramie

Calli were produced from cotyledons of ramie variety Liuyang Daleyu on MSB agar medium containing 0. 5 mg/L 2, 4-D and 0. 5 mg/L KT. After subculture for 3 - 4 times, the calli were shaken in the MSB liquid medium and then formed suspension cell lines. Protoplast were isolated from the cells. Only when cultured on KM8P medium with alginate embedding, can they grow vigorously and form microcalli within fifty days. Protoplast-derived calli were subcul-tured on the MSB agar medium with 0. 2 mg/L 2, 4-D and 0. 1 mg/L 6-BA and then transferred onto MSB medium containing 2. 0 mg/L 6-BA to induce shoots. After reaching 2 cm in height of shoots they were transferred onto H medium with 0. 05 mg/L NAA. Root formation was induced and therefore complete plants were obtained.