西北旱区枣疯病植原体核糖体蛋白基因的生物信息学分析

枣疯病是枣树上的一种具有毁灭性的植原体病害,几乎分布于国内所有的枣树栽培区,造成了巨大的经济损失.对我国陕西、宁夏、甘肃3省枣疯病样品植原体核糖体蛋白基因进行克隆和测序,获得枣疯病植原体的核糖体基因片段为1 196bp,包含部分rps19,rpl22和rps3三个基因,其中rpl22和rps3大小分别354bp和753bp,分别编码118和251个氨基酸,且这两个基因为非重叠基因.序列同源性比较结果表明:我国陕西、宁夏、甘肃的枣疯病植原体的核糖体蛋白rp基因大小一致,归属于植原体16S rⅤ-B组;该植原体核糖体蛋白基因特性与樱桃致死黄化(CLY5)和桃树黄化印度分离株系(PY-In)植原体相似.首次报道了我国枣疯病核糖体蛋白基因rp基因的序列,把枣疯病植原体归到16S rⅤ-B组,为枣疯病植原体提供了新的分类依据.

Bioinformatics analysis of ribosomal protein gene of JWB phytoplasma in the northwestern arid regions

Jujube witches'' broom (JWB) is a destructive disease caused by phytoplasma , that happened almost in every cultural regions at home and abroad , bringing large economic loss. The ribosomal protein (p) gene is cloned by polymerase chain reaction from the samples of JWB which were collected from Shaanxi, Ningxia and Gansu. After se-quenced, it is found that p gene is 1199 bp, which included partial rps19, rpl22 and rps3. rpl22 and rps3 coded 129 and 252 amino acids respectively, and these two genes are not overlapped . The homologic analysis shows that pathogens from Shaanxi, Ningxia and Gansu are same; and it is similar in character among JWB, cherry lethal yellows and peach yellows isolate PY-In phytoplasmas . This study reports rp sequences of JWB phytoplasmas for the first time and identi-fies that JWB phytoplasma belongs to 16S rV-B, which can provide theory basis for research of the functional sec system of JWB phytoplasma.