牛、羊卵母细胞孤雌激活的研究

本实验主要比较了离子霉素、电脉冲2种方法激活牛、羊体外成熟卵母细胞的效率。2种激活方法中,牛胚胎卵裂率无显著差异(90.61%VS94.40%,P>0.05),而离子霉素激活胚胎的囊胚发育率极显著高于电激活方法(12.3%VS2.4%,P<0.01)。2种方法对羊的胚胎的研究中,羊胚胎卵裂率无显著差异(72.4%VS77.4%,P>0.05)。但是离子霉素激活胚胎的囊胚发育率显著高于电激活方法(3.67%VS10.4%,P<0.05)。本实验中还比较了用化学激活(离子霉素)激活牛体外成熟卵母细胞后,用SoFaa体系培养,换液与不换液对孤雌激活胚胎体外发育的影响。结果表明:在第四天不换液的胚胎卵裂率和囊胚率极显著高于换液的胚胎(11.64%VS3.49%,P<0.01)。

Improvement of Acid A-napHtyl Acetate Esterase Staining Study on bovine/ovine oocyte parthenogenetic activation

The parthenogenesis efficiencies of bovine in vitro maturation (IVM) oocytes were also studied between chemical and electric activation. The effect of different culture method on the development of parthenogenetic embryos from chemical activation was investigated in Synthetic Oviduct Fluid (SOF) culture system. There were no significant difference in the cleavage rate(90.61% vs 94.40%, P>0.05) between the two methods, but the blastocyst rate in chemical activation group was significantly higher than that of the electric activation (12.30% vs 2.4%,p<0.01). There were no significant difference in the cleavage rate of ovine embryo between the two methods(72.4% VS 77.4%,P>0.05), but the blastocyst rate in chemical activation group was significantly higher than that of the electric activation (3.67% VS 10.4%,P<0.05). Two methods were used for chemical activation parthenogenetic embryos culture. One was the activated bovine IVM oocytes cultured in the SOFaa medium for 7 days, which we stated as method A. Another was the activated bovine IVM oocytes cultured in the SOFaa medium for 4 days, then transferred into the SOFaa medium supplemented with 10% FBS for 3 days, which we stated as method B. The result showed that the blastocyst rates were significantly different between method A and method B (11.64% vs 3.49%,p<0.01).