| 斜纹夜蛾乙酰胆碱酯酶基因cDNA片段的克隆和序列分析 |
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| 引用本文： | 陈亮,李兵,浦冠勤.斜纹夜蛾乙酰胆碱酯酶基因cDNA片段的克隆和序列分析[J].蚕业科学,2010,36(1):138-142. |
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| 作者姓名： | 陈亮 李兵 浦冠勤 |
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| 作者单位： | 苏州大学金螳螂建筑与城市环境学院,江苏苏州,215123;苏州大学基础医学与生物科学学院,江苏苏州,215123 |
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| 基金项目： | 国家重点基础研究发展计划“973”项目(No.2005CB12-1005);;苏州市科技支撑计划项目(No.SNG0924) |
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| 摘 要： | 斜纹夜蛾(Spodoptera litura)是桑树的主要害虫之一。昆虫体内的乙酰胆碱酯酶(AChE,EC3.1.1.7)是有机磷和氨基甲酸酯类杀虫剂的作用靶标。通过设计简并引物,利用RT-PCR技术克隆了斜纹夜蛾的乙酰胆碱酯酶基因cDNA片段(GenBank登录号:FJ959384)。序列分析结果表明,该cDNA片段全长1 191 bp,编码397个氨基酸,包含了乙酰胆碱酯酶基因的特征位点区域。基于乙酰胆碱酯酶氨基酸同源序列的进化分析表明,斜纹夜蛾与甜菜夜蛾(Spodoptera exigua)的亲缘关系最近,与棉蚜(Aphis gossypii)的亲缘关系最远。该基因cDNA片段的克隆有助于对斜纹夜蛾乙酰胆碱酯酶结构、功能及抗药性的分子机制研究。
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| 关 键 词： | 斜纹夜蛾 乙酰胆碱酯酶 基因克隆 反转录PCR 序列分析 |
Cloning and Sequence Analysis of cDNA Fragment of Acetylcholinesterase Gene in Spodoptera litura |
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| CHEN Liang,LI Bing,PU Guan-Qin.Cloning and Sequence Analysis of cDNA Fragment of Acetylcholinesterase Gene in Spodoptera litura[J].Acta Sericologica Sinica,2010,36(1):138-142. |
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| Authors: | CHEN Liang LI Bing PU Guan-Qin |
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| Institution: | 1Golden Mantis School of Arichitecture and Urban Environment;Soochow University;Suzhou Jiangsu 215123;China;2School of Basic Medicine and Biological Sciences;China |
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| Abstract: | Spodoptera litura is one of the major mulberry pests.Insect acetylcholinesterase(AChE,EC3.1.1.7) is the target of organophosphorus and carbamate insecticides.By means of RT-PCR,a cDNA sequence of the acetylcholinesterase gene(GenBank accession number FJ959384) in the Spodoptera litura was cloned by using degenerate primers.Restriction enzyme digestion and sequence analysis showed that the obtained fragment is 1 191 bp long and has an open reading frame encoding a 397 amino acid protein which has the charact... |
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