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| 鸭坦布苏病毒E蛋白中和性单克隆杭体的制备 | |
| 引用本文： | 姬希文,李雪松,李国新,肖亚莉,颜丕熙,徐大伟,范钊,张七斤,李泽君.鸭坦布苏病毒E蛋白中和性单克隆杭体的制备[J].中国家禽,2012,34(4):25-28,32. |
| 作者姓名： | 姬希文 李雪松 李国新 肖亚莉 颜丕熙 徐大伟 范钊 张七斤 李泽君 |
| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特010018;中国农业科学院上海兽医研究所,农业部寄生虫重点开放实验室,上海200241 2. 中国农业科学院上海兽医研究所,农业部寄生虫重点开放实验室,上海200241 3. 内蒙古农业大学兽医学院,内蒙古呼和浩特,010018 4. 中国农业科学院上海兽医研究所,农业部寄生虫重点开放实验室,上海200241;国家家禽工程技术研究中心,上海200241 |
| 基金项目： | 国家自然科学基金面上项目，公益性行业(农业)科研专项经费项目，863计划，国际合作项目 |
| 摘 要： | 本试验利用纯化的鸭坦布苏病毒(Duck Tembusu virus , DTMUV)奉贤株(FX2010)免疫BALB/c小鼠,通过淋巴细胞杂交瘤技术,筛选获得3株能够稳定分泌杭DTMUV的杂交瘤细胞,分别命名为:J1-E5-E4, 7B5和1E5-B6,3株杂交瘤细胞诱导同品系刁、鼠的杭体效价为1:12 800,1:6 400和1:25 600。间接ELISA和IFA结果显示,3株单杭均可以与DTMUV发生特异性反应。进一步研究发现,J1-E5-E4可以与DTMUV-E蛋白结合,使得表达E蛋白的293T细胞显现出特异性的绿色荧光;在病毒中和试验中,J1-E5-E4可以中和DTMUV,使其失去致死鸡胚的能力。这些结果表明,J1-E5-E4是一株针对DTMUV-E蛋白中和表位的单杭,该单杭将在DTMUV诊断和杭原分析中发挥重要作用。 |
| 关 键 词： | DTMUV E蛋白 中和 单克隆抗体 |
Development of Neutralizing Monoclonal Antibody Against Protein E of Duck Tembusu Virus |
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| JI Xiwen,LI Xuesong,LI Guoxin,XIAO Yali,YAN Pixi,XU Dawei,FAN Zhao,ZHANG Qijin,LI Zejun.Development of Neutralizing Monoclonal Antibody Against Protein E of Duck Tembusu Virus[J].China Poultry,2012,34(4):25-28,32. | |
| Authors: | JI Xiwen LI Xuesong LI Guoxin XIAO Yali YAN Pixi XU Dawei FAN Zhao ZHANG Qijin LI Zejun |
| Institution: | 2,3(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018;2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241;3.National Poultry Engineering and Technology Research Center,Shanghai 200241) |
| Abstract: | BALB/c mice were immunized with the purified Fengxian strain(FX2010) of duck Tembusu virus(DTMUV).Three monoclonal antibodies(McAb) designated J1-E5-E4,7B5 and 1E5-B6 were obtained by lymphocyte hybridoma technique.The ascites titers of J1-E5-E4,7B5 and 1E5-B6 were 1:12 800,1:6 400 and 1:25 600,respectively.All three McAbs were able to react with DTMUV specifically by indirect ELISA and indirect immunofluorescence assay(IFA).Importantly,J1-E5-E4 was found to bind to protein E of DTMUV specifically in cells and neutralize DTMUV in chicken embryos.The McAb would be useful in clinical diagnosis and antigen analysis of DTMUV. |
| Keywords: | DTMUV protein E neutralization monocolnal antibody |
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