Monitoring genetic stability inQuercus serrata Thunb. somatic embryogenesis using RAPD markers |
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| Authors: | Ramesh C. Thakur Susumu Goto Katsuaki Ishii S. Mohan Jain |
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| Affiliation: | (1) Forestry and Forest Products Research Institute, 305-8687 Tsukuba, Ibaraki, Japan;(2) Fukuoka Prefecture Forest Research and Extension Center, 839-0827 Kurume, Fukuoka, Japan;(3) University of Helsinki, 00014, Finland |
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| Abstract: | Genetic stability of propagules regeneratedvia somatic embryogenesis is of paramount importance for its application to clonal forestry. Random amplified polymorphic DNA (RAPD) markers were used to determine the genetic stability in somatic embryogenesis ofQuercus serrata Thunb. (Japanese white oak). Forty samples from an embryogenic line, consisting of regenerated plantlets, somatic embryos, and embryogenic calli, were examined using 54 decanucleotide primers. A total of 6520 clear reproducible bands obtained from these studies exhibited no aberration in RAPD banding pattern among the tested samples. Our results show that somaclonal variation is absent in our plant propagation system. The genetic stability is discussed in terms of the origin of somatic embryos. |
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| Keywords: | genetic stability Japanese white oak Quercus serrata RAPD somatic embryogenesis |
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