| 裂叶秋海棠组织培养及植株再生研究 |
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| 引用本文： | 陈刚,梁晶龙,陈雄伟,金红. 裂叶秋海棠组织培养及植株再生研究[J]. 北方园艺, 2009, 0(6) |
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| 作者姓名： | 陈刚 梁晶龙 陈雄伟 金红 |
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| 作者单位： | 肇庆学院,生命科学学院,广东,肇庆,526061;深圳市仙湖植物园科技部,广东,深圳,518004 |
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| 基金项目： | 广东省自然科学基金资助项目(2007B020704001);;肇庆学院教学研究基金资助项目(0629) |
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| 摘 要： | 以裂叶秋海棠（Begonia palmata D.Don）种子获得无菌苗,取其叶片为外植体,对裂叶秋海棠叶片丛生芽诱导和植株再生进行了研究。结果表明：MS＋TDZ 0.5 mg/L诱导叶片外植体产生不定芽的诱导效果最佳,诱导率为100%,不定芽个数可达200个/cm2;最佳生根培养基为1/3MS＋IBA 0.2 mg/L,2周后生根率达到100%。
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| 关 键 词： | 裂叶秋海棠 组织培养 植株再生 |
Study on Tissue Culture and Plantlet Regeneration of Begonia palmata D. Don |
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| CHEN Gang,LIANG Jing-long,CHEN Xiong-wei,JIN Hong. Study on Tissue Culture and Plantlet Regeneration of Begonia palmata D. Don[J]. Northern Horticulture, 2009, 0(6) |
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| Authors: | CHEN Gang LIANG Jing-long CHEN Xiong-wei JIN Hong |
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| Affiliation: | 1.Faculty of Life Science;Zhaoqing University;Zhaoqing;Guangdong 526061;China;2.Department of Science and Technology;Shenzhen Fairylake Botanical Garden;Shenzhen;Guangdong 518004;China |
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| Abstract: | Using tissue culture methods, different set of hormone concentration of medium cleft leaf begonia (Begonia palmata D. Don)leaves were studied. Results showed that MS+6-BA 2.0 mg/L and MS+4-PU 0.5 mg/L induced leaf explants of adventitious buds produced the best results induction, induction rate was 100%, Bud were 150/cm2, and 120/cm2; one of three strength MS was the best medium for rooting,and rooting rate reached 100% after two weeks. |
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| Keywords: | Cleft leaf begonia Tissue culture Regeneration |
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