RAPD genetic diversity of Albanian olive germplasm and its relationships with other Mediterranean countries |
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Authors: | Angjelina Belaj Zlatko Satovic Hajri Ismaili Dhimitër Panajoti Luis Rallo Isabel Trujillo |
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Institution: | 1. Departamento de Agronomía, ETSIAM, Universidad de Córdoba, Avenida Ménendez Pídal s/n, Apdo 3048, 14080, Córdoba, Spain 2. Faculty of Agriculture, University of Zagreb, Department of Seed Science and Technology, Svetosimunska 25, 10000, Zagreb, Croatia 3. Institute of Arboriculture, Uj?t e Ftoht?, Vlor?, Albania
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Abstract: | RAPD markers were used for the study of 19Albanian olive cultivars and two wild olives (oleasters). A total of 76polymorphic
bands (4.8 polymorphic markers per primer) out of 107 reproducible were obtained using 16 primers. The number of bands per
primer ranged from 4 to 10,whereas the number of polymorphic bands ranged from 1 to 9, corresponding to 71%of the total amplification
products. All the accessions could be identified by the combination of four primers: OPA-19;OPA-02; OPK-16 and OPP-19. The
dendrogram,based on Jaccard's index, included three major groups according to their origin: 1)most of the cultivars from the
area of Berat (South of Albania) 2) cultivars from the Centre and Centre-North of Albania and3) cultivars from the Centre
and North-West of Albania along with the oleaster from Elbasan. In order to evaluate the origin of Albanian cultivars they
were compared to those diffused in other countries like Greece, Italy and Turkey, due to geographical and historical affinity
among these countries, by using a one way AMOVA. Although most of the genetic diversity was attributable to differences among
cultivars within each country (91.47%) significantφ-values among countries(φst = 0.085; p < 0.001)suggested the existence of RAPD phenotypic differentiation. Significant φ-values in all pairs formed by Albania with
the other countries were observed. These results are consistent with the autochthonous origin of Albanian cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | AMOVA DNA fingerprinting genetic resources Olea Europaea L oleaster |
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