New ELISA test for detection of bovine leukemia virus infections in cattle, using bacterially synthesized p24 |
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| Authors: | H Siakkou D Kube H Peters A Otto R Ulrich S Rosenthal |
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| Affiliation: | Department of Cell Differentiation, Academy of Sciences, Berlin-Buch. |
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| Abstract: | A new ELISA test is described for the detection of antibodies to bovine leukemia virus protein p24. This test employs a bacterially synthesized p24 antigen which represents a hybrid protein consisting of beta-galactosidase and about 70% of the mature viral p24. The antigen preparation was enriched from Escherichia coli cells to 95% purity and was used for the detection of antibodies in cattle. In a selected set of 100 positive field sera, 97 could be verified by the new test. |
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