| 星形孢菌素产生菌的选育研究 |
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| 引用本文： | 蒲小明,林壁润,胡美英,沈会芳.星形孢菌素产生菌的选育研究[J].核农学报,2008,22(3):276-279. |
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| 作者姓名： | 蒲小明 林壁润 胡美英 沈会芳 |
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| 作者单位： | 1. 广东省农业科学院植物保护研究所,广东广州,510640
2. 华南农业大学,广东广州,510640 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家自然科学基金 |
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| 摘 要： | 用紫外线和微波复合诱变星形孢菌素产生菌(Stretomyces sp.4138),以硫酸链霉素抗性偶联进行筛选。结果表明:紫外线处理50s后,在含硫酸链霉素0.8μg/ml的分离培养基上筛选到突变株HZ-09,比原始菌株H-0效价提高了30.46%;再以HZ-09为出发菌株进行微波处理30s后,硫酸链霉素抗性筛选获得突变株HW-25,比原始菌株H-0效价提高了284.20%。菌株HW-25经多次传代,遗传性状稳定。
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| 关 键 词： | 紫外线 微波 硫酸链霉素 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
SCREENING OF STAUROSPORINE-PRODUCING MUTANT STRAIN |
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| PU Xiao-ming,LIN Bi-run,HU Mei-ying,SHEN Hui-fang.SCREENING OF STAUROSPORINE-PRODUCING MUTANT STRAIN[J].Acta Agriculturae Nucleatae Sinica,2008,22(3):276-279. |
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| Authors: | PU Xiao-ming LIN Bi-run HU Mei-ying SHEN Hui-fang |
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| Abstract: | A staurosporine-producing strain Stretomyces sp.4138 was treated with ultraviolet and microwave,and then selected by resistant combined material streptomycin sulfate.With 50 seconds ultraviolet treatment,a mutant strain HZ-09 was obtained after plate screening on the isolation medium plus(streptomycin sulfate 0.8μg/ml).The titer of HZ-09 was 30.46% higher than the parent strain H-0.Then the strain HZ-09 was treated 30s by microwave and combined with streptomycin sulfate to get a mutant strain HW-25,whose titer was 284.20% higher than that of the original H-0.The high productivity of the strain HW-25 could be inherited stably. |
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| Keywords: | ultraviolet microwave streptomycin sulfate |
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