中国主要产区何首乌以二苯乙烯苷含量为指标的种质评价

采集我国几大主要产区的野生何首乌及部分栽培品，采用高效液相色谱法(HPLC)对其特征性成分2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量进行测定，分析了6个野生居群和2个栽培居群以及同一居群同一生长环境不同年限的样品和同一居群同一年限在不同环境条件下生长的样品。实验结果表明：采自广西的一种何首乌药材原植物是何首乌的一个变种棱枝何首乌(P.multiflorum Thunb.var angulatum S.Y.Liu)，而且其中2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷含量较低；贵州、湖北居群2，3，5，4’-四羟基二苯乙烯-2一O—B—D-葡萄糖苷含量较高，四川、河南较低；另外还发现，种质的不同，年限的差异及不同环境是影响2，3，5，4’-四羟基二苯乙烯-2-O—β—D-葡萄糖苷积累的重要因素。

GERM PLASM APPRAISEMENTS OF POLYGONUM MULTIFLORUM FROM MAIN PRODUCING AREAS IN CHINA BY CONTENT OF TETRAHYDROXYSTILBENE

Six wild and 2 cultivated populations of Polygonum multiflorum Thunb.(heshouwu)were collected from main producing areas in China.HPLC was used to determine content of the characteristic component 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in root in order to appraise their germ plasms.Results of the study showed that,the sample from Guangxi Province is a variety of P.multiflorum Thunb.namely P.multiflorum Thunb.var angulatum S.Y.Liu,and its content of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside is relatively low.2,3,5,4′-Tetrahydroxy-stilbene-2-O-β-D-glucoside contents of samples from Guizhou and Hubei are higher,while those from Sichuan and Henan are lower.It is also found that location,age and environment are the three important factors determining the accumulation of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in the plant.