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| 引用本文： | 赵永红,李 婵,梅嘉洺,王 咏,朱美兰,阳宴清,卢运海.索引[J].热带作物学报,2016(2):29-30. |
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| 作者姓名： | 赵永红 李 婵 梅嘉洺 王 咏 朱美兰 阳宴清 卢运海 |
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| 作者单位： | 1 福建农林大学作物科学学院 福建福州 350002；
2 国家菌草工程技术研究中心 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002；
2 国家菌草工程技术研究中心 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002；
2 国家菌草工程技术研究中心 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002;1 福建农林大学作物科学学院 福建福州 350002 |
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| 基金项目： | 基金项目：国家菌草工程技术研究中心攻关课题项目(No. JCGG14010)。 |
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| 摘 要： | 利用SCoT分子标记对47个菌草种质资源进行遗传多样性分析。从20个SCoT引物中筛选出10个扩增效果好的引物，对47份菌草种质材料的DNA进行PCR扩增,共获得282个多态片段。结合UPGMA聚类分析方法，算出47份种质材料间的遗传相似系数(SM)在0.77-0.96，所有47份材料被区分开来，在遗传相似系数为0.815时，47份材料可分为5大类群。研究结果表明，SCoT分子标记技术可有效用于菌草资源的遗传多样性分析。
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| 关 键 词： | 菌草 SCoT分子标记 种质资源 遗传多样性 |
Genetic Diversity of Juncao Germplasms by SCoT Markers |
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| ZHAO Yonghong,LI Chan,MEI Jiaming,WANG Yong,ZHU Meilan,YANG Yanqing and LU Yunhai.Genetic Diversity of Juncao Germplasms by SCoT Markers[J].Chinese Journal of Tropical Crops,2016(2):29-30. |
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| Authors: | ZHAO Yonghong LI Chan MEI Jiaming WANG Yong ZHU Meilan YANG Yanqing and LU Yunhai |
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| Institution: | 1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;
2 National Engineering Research Center of Juncao Technology, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;
2 National Engineering Research Center of Juncao Technology, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;
2 National Engineering Research Center of Juncao Technology, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002;1 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002 |
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| Abstract: | The genetic diversity of 47 Juncao germplasms was analyzed by SCoT markers. Twenty ScoT primers were first used for screening on a subset of four germplasms, of which 10 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. A total of 282 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 10 selected SCoT primers. By using the NTSYS-pc 2.1 software combined with UPGMA clustering analysis method, the genetic similarity coefficient (SM) was calculated among all accessions and ranged from 0.77 to 0.96. All the 47 accessions were distinguished from each other. The 47 JUNCAO germplasms were clustered into five groups when the genetic similarity coefficient was 0.815. Our results showed that the SCoT markers could be effectively used for analysis of genetic diversity of Juncao germplasms. |
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| Keywords: | Juncao ScoT Germplasm Genetic diversity |
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