利用间歇浸没式植物生物反应器培养金钗石斛种苗

以金钗石斛（Dendrobium nobide Lindl.）原球茎为材料，利用间歇浸没式植物生物反应器进行金钗石斛组培快繁的研究。通过优化浸没频率、接种量和培养基蔗糖浓度，获得最佳的培养条件为：浸没频率5 min/8 h、接种量10 g·L-1、蔗糖浓度20 g·L-1，并与传统的固体培养比较，反应器培养在组培苗的生长形态、增殖倍数和有效成分上都明显优于固体培养。利用间歇浸没式植物生物反应器进行金钗石斛种苗培养，为金钗石斛种苗的高效率、低成本的生产提供了一种新的方法。

Micropropagation of Dendrobium nobile Seedlings Using Temporary Immersion Bioreactor System

Taking the protocorm of Dendrobium nobile Lindl. as material, the tissue culture and rapid propagation of D. nobile were studied in the temporary immersion bioreactor. The optimum culture conditions were obtained: the immersion frequency was 5 min / 8 h, the inoculation amount was 10 g·L-1 and the sucrose concentration was 20 g·L-1. Compared with the traditional solid culture, the bioreactor culture was superior to the solid culture in the growth morphology, multiplication rate and effective components of the tissue culture seedlings, which provided a new method for the production of D. nobile seedlings with high efficiency and low cost.