白血病抑制因子（LIF）及其受体在月经 周期猕猴卵巢内的表达

应用免疫细胞化学方法对白血病抑制因子（leukemia inhibitory factor, LIF）、白血病抑制因子受体（leukemia inhibitory factor receptor, LIFR）和gp130在月经周期猕猴卵巢内的表达进行了研究。结果表明：LIF、LIFR和gp130在猕猴卵巢内的表达量随月经周期的不同而变化，LIF及其受体在增殖期卵巢内的表达量高于分泌期的卵巢。LIF及其受体在原始卵泡、腔前卵泡和有腔卵泡的卵细胞内表达量均较高，在颗粒细胞、卵泡膜、卵巢生殖上皮和卵巢基质中有少量表达，而在闭锁卵泡、退化的黄体和卵巢髓质中未见表达。LIF及其受体在猕猴卵巢内的表达可能受卵巢分泌的类固醇激素所调控，LIF可能通过自分泌或旁分泌的方式在猕猴卵泡发育及排卵等过程中起重要作用。

Leukemia Inhibitory Factor and Its Receptors in Rhesue Monkey Ovary during the Menstrual Cycle

This study was done to examine immunohistochemically the presence of LIF, LIFR and gp130 in the monkey ovary during the menstrual cycle. The results showed that the immunostaining for LIF, LIFR and gp130 was cycle-dependent, was considerably higher during proliferative phases than during secretory phases of the menstrual cycle. The oocytes at all stages of development were the primary site of immunoreactive LIF, LIFR and gp130, which were present to a lesser extent in the granulose cells, theca cells, stromal cells and serosal tissue. No immunostaining for LIF, LIFR and gp130 was found in atretic follicles, regressed corpora lutea. The cycle dependency suggests a potential regulation of their expression by ovarian steroids. LIF may play an important role in the regulation of monkey follicular development and ovulation.