| 提高甜菜遗传转化频率的研究 |
| |
| 引用本文： | 张海霞,张少英,白薇,李会东.提高甜菜遗传转化频率的研究[J].中国糖料,2005(1):6-8. |
| |
| 作者姓名： | 张海霞 张少英 白薇 李会东 |
| |
| 作者单位： | 1. 内蒙古农业大学甜菜生理研究所,呼和浩特,010018
2. 湖南科技大学化学化工学院,湘潭,411201 |
| |
| 基金项目： | 国家自然科学基金资助项目(No.30060048) |
| |
| 摘 要： | 以甜菜叶柄为外植体，通过农杆菌介导转化，将玉米胚乳核糖体失活蛋白基因(cRIP)导入甜菜，对影响转化频率的因素：卡那霉素的浓度、头孢霉素的浓度、农杆菌感染时间和浓度等进行了探讨，初步建立了转化频率较高的转化系统．当卡那霉素浓度为100mg／L，头孢霉素浓度为500mg／L，农杆菌浓度OD值为0.3-0．4，感染时间为8～10min时．抗性不定芽分化率可达36．5％。该转化系统为转基因植株的获得奠定了基础。
|
| 关 键 词： | 甜菜 遗传转化 频率 |
| 文章编号： | 1007-2624(2005)01-0006-03 |
| 修稿时间： | 2004年9月6日 |
Study on Improvement of Transformation Frequency of Sugarbeet Mediated by Agrobacterium |
| |
| ZHANG Hai-xia,ZHANG Shao-ying,BAI Wei,LI Hui-dong.Study on Improvement of Transformation Frequency of Sugarbeet Mediated by Agrobacterium[J].Sugar Crops of China,2005(1):6-8. |
| |
| Authors: | ZHANG Hai-xia ZHANG Shao-ying BAI Wei LI Hui-dong |
| |
| Institution: | ZHANG Hai-xia1,ZHANG Shao-ying1,BAI Wei1,LI Hui-dong2 |
| |
| Abstract: | cRIP genes were transfered into sugarbeet via Agrobacterium using petioles as explants. The factors that may affect transformation frequency were investigated, such as concentration of Kanamycin(Kan) and Cefazolin sodium(Cef), infection time and infection concentration of Agrobacterium. When infection concentration was OD=0.3-0.4 and infection time was 8-10 minutes while 100mg/L Kan and 500mg/L Cef were added into the selective medium, high transformation frequency can be obtained. A stable and efficient Agrobacterium mediated transformation system has been established. |
| |
| Keywords: | Surgarbeet Genetic transformation Frequency |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
