rDNA polymorphism of foxtail millet (Setaria italica ssp. italica) landraces in northern Pakistan and Afghanistan and in its wild ancestor (S. italica ssp. viridis) |
| |
| Authors: | Kenji Fukunaga Katsuyuki Ichitani and Makoto Kawase |
| |
| Institution: | (1) Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka-cho 562 Shobara, Hiroshima 727-0023, Japan;(2) Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, Kagoshima Kagoshima, 890-0065, Japan;(3) Genebank, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba Ibaraki, 305-8602, Japan |
| |
| Abstract: | Ribosomal DNA (rDNA) spacer length polymorphism was studied in foxtail millet (Setaria italica ssp. italica) landraces from Pakistan and Afghanistan and in its wild ancestor (S. italica ssp. viridis) from Pakistan by PCR-based methods. Sequence polymorphism was also investigated for accessions selected based on the observed
length polymorphism. The PCR-based length polymorphism and sequence polymorphism of rDNA intergenic spacer (IGS) clearly demonstrated
genetic differentiation between cultivated and wild forms in the region. Genetic differentiation was observed between different
areas to some extent in the cultivated form, and between different regions in the wild form of northern Pakistan. Based on
the results, we discuss the genetic differentiation of foxtail millet and wild ancestor in this region and possible utility
of rDNA markers to trace the dispersal of this crop in the region. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
