利用RAPD分析药用石斛的遗传多样性及亲缘关系

通过对9种药用石斛进行遗传多样性和亲缘关系的分析,为更好地开发利用石斛资源奠定基础。采用RAPD技术,应用NTSYS软件对9种石斛的RAPD-PCR结果进行分析。利用从103条随机引物中筛选出的70条随机引物对供试材料DNA进行随机扩增,共得到520条带,其中多态性带有491条,多态性百分率为94.42%。采用UPGMA类平均法对扩增出的谱带进行遗传聚类分析,得出反映各种间亲缘关系的树状图。在遗传距离（D）=0.44处,可将供试材料分为2类,RAPD对基因组的分析结果与传统分类学的结果差异不大。试验结果表明,RAPD技术可有效地应用于药用石斛的遗传多样性及亲缘关系的研究。

Analysis of Genetic Diversity and Affinity Relationships Among the Medical Species of Dendrobium Sw. by RAPD

Genetic diversity and affinity relationships among 9 medical species of Dendrobium Sw.were analyzed,the result laid a solid foundation for the better use of this resources.The genetic diversity of 9 species Dendrobium Sw.was tested by using RAPD(random amplified polymorphic DNA) technique.The NTSYS software was used to analyze the markers.Seventy RAPD primers from 103 random primers were applied to do random amplification.A total of 520 DNA bands was detected,471 among which were polymorphic,the average rate of polymorphic bands was 94.42%.The result of cluster analysis by using UPGMA method showed that 9 genotypes could be classified into two types in genetic distance 0.44.This outcome was corresponding to the result by using traditional classification.Conclusion: The result of this research showed that RAPD molecular marker is efficient to study the medical Dendrobium Sw.genetic diversity.