Molecular identification and characterization of a new phytoplasma strain associated with Chinese chestnut yellow crinkle disease in China |
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| Authors: | C L Lin H F Li G Z Zhang W Wei X Q Zhu Z P Li H Wang Q C Xu T Zhou G Z Tian |
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| Institution: | 1. Key Laboratory of Forest Protection of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China;2. Department of Plant Pathology, China Agricultural University, Beijing 100193, China;3. Molecular Plant Pathology Laboratory, USDA‐Agricultural Research Service, Beltsville, MD 20705, USA;4. Forest Pest and Disease Control and Quarantine Station of Huairou District, Beijing Forestry Bureau, Beijing, 101400, China;5. Forest Protection Station, Beijing Forestry Bureau, Beijing 100029, China;6. E‐mail: tiangz@caf.ac.cn (for correspondence) |
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| Abstract: | Chinese chestnut trees (Castanea mollissima BL.) planted in a suburb of Beijing, China developed symptoms including yellowing, leaf crinkling, little leaf, shortened internodes, and empty burrs. Transmission electron microscopy revealed presence of phytoplasma cells in phloem sieve elements of the symptomatic chestnut trees. Molecular cloning and sequence analysis of PCR‐amplified near‐full length 16S rRNA gene indicated that the phytoplasma associated with the Chinese chestnut yellow crinkle disease is closely related to Japanese chestnut witches’‐broom phytoplasma. This is the first report of a phytoplasmal disease in Chinese chestnut trees. |
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