绵羊肺炎支原体贵州株P113基因的克隆与生物信息学分析

为获得绵羊肺炎支原体贵州株P113基因生物信息学特征,应用DNAStar、Mega 5.0、Protparam、Protscale、IEBD等工具对其(GZ-QX1株)P113蛋白特性、结构和功能进行预测分析。结果显示,绵羊肺炎支原体GZ-QX1株P113基因序列大小为3 240bp,编码1 079个氨基酸,与绵羊肺炎支原体Y98标准株、四川SC01株、猪肺炎支原体P97、丝状支原体山羊亚种、山羊支原体山羊亚种的核苷酸序列同源性分别为99.9%、81.9%、60.4%、3.9%和5.2%。P113蛋白是分子质量约119ku的碱性蛋白,具有较多优势抗原表位;蛋白结构分析显示,P113蛋白无跨膜结构,有9个N糖基化位点,59个丝氨酸、16个苏氨酸的磷酸化位点,14种保守的特异性蛋白质激酶的结合位点;蛋白功能分析认为,P113可能是某信号传导通路的信号分子,也是一种具有良好抗原性的结构蛋白。

Clone and Bioinformatics Analysis of P113 Gene of Mycoplasma ovipneumoniae in Guizhou Province

The assay was aimed to access the biological characteristics of P113 protein in Mycoplasma ovipneumoniae(Mo) Guizhou strain (GZ-QX1). The protein structure,characteristics and function of GZ-QX P113 protein were predicted and analyzed by DNASTAR,MEGA 5.0 softwares,Protparam,Protscale and IEBD online tools in this study. The results showed that Mo GZ-QX1 P113 gene predicted is 3 240 bp,encoding 1 079 amino acid(AA),and sharing nucleotides sequence identity 99.9% with Mo Y98 standard strains 81.9% with SC01 Sichuan strain,60.4% with Mycoplasma hyopneumoniae P97, 3.9% with Mycoplasma mycoides and 5.2% with Mycoplasma capripneumoniae. The molecular weight of P113 protein is 119 ku,with more advantages antigen epitope protein structure. Protein structure analysis showed that the protein had no transmembrane domain. There were 9 N-glycosylation sites,59 serine,16 threonine may be phosphorylated predicted and 14 conserved and specific phosphkinase binding sites. Protein functional analysis showed that P113 may function as a mediator in signaling pathway,but also a kind of good antigenic structure of proteins.