果桑种质资源SRAP指纹图谱构建及遗传差异分析

为了有效区分15份果桑种质资源，利用分子标记SRAP技术进行遗传差异分析并构建DNA指纹图谱。从42对SRAP引物组合中筛选出17对引物进行PCR扩增，得到306条清晰条带，其中多态性条带253条，多态性比率为82.68%，供试材料间的遗传相似系数(GS)在0.390 9～0.811 1之间。选用2对多态性引物（Me2/Em1 和Me7/Em5），初步构建了15份果桑种质材料的DNA指纹图谱。经过非加权组平均法(UPGMA)聚类分析，以遗传相似系数0.666为阈值，将供试材料分为3组；根据条带的有无转换为二进制编码形成数字指纹图谱，简便快速区分每份种质材料。采用SRAP分子标记建立的指纹图谱适合于果桑品种的分类和鉴定。

Establishment of Fingerprint and Analysis of Genetic Difference for Fruit Mulberry Germplasm Resources by SRAP Markers

In order to effectively distinguish 15 fruit mulberry germplasm materials,SRAP markers were employed to analyze the genetic differences and to develop their DNA fingerprints.Seventeen pairs of primers were selected from 42 pairs of SRAP primers,and 306 bands were amplified including 253 polymorphic bands.The percentage of polymorphic bands was 82.68%.The genetic similarity coefficients (GS) among materials ranged from 0.390 9 to 0.811 1.Two pairs of polymorphic primers of Me2/Em1 and Me7/Em5 were used to develop the DNA fingerprints for 15 fruit mulberry germplasm materials.Unweighted pair-group method arithmetic average (UPGMA) was used to develop the dendrogram and all materials were divided into three major groups at the 0.666 similarity level.The DNA fingerprints were then converted into binary codes based on band presence or absence.In the DNA fingerprints,each of fruit mulberry germplasm material had its unique binary code which could be distinguished between each other.The results indicated that DNA fingerprints established by SRAP markers was suitable for distinguishing fruit mulberry cultivars.