大孔吸附树脂富集·纯化粟米草总皂苷工艺研究

目的]研究大孔吸附树脂富集、纯化粟米草总皂苷的工艺。方法]利用大孔吸附树脂技术，通过对4种不同型号（AB-8、D101、HPD100、HPD600）的树脂进行选择，采用静态吸附方法确定适合的树脂型号；采用动态吸附方法，以总皂苷的得率为指标，考察其纯化富集的工艺条件。结果]4种树脂型号中，D101型大孔吸附树脂对粟米草总皂苷具有良好的纯化富集作用，对粟米草总皂苷的洗脱率达80．9％，其工艺条件为：洗脱溶剂用体积分数为60％的乙醇，料液质量浓度为0．5g/ml，洗脱速率为2BV／h，树脂药材质量比2：1。结论]用D101型大孔吸附树脂富集和纯化粟米草总皂苷的工艺具有吸附量较大、洗脱率较高、树脂再生简便等优点，在粟米草皂苷的富集纯化和生产中具有推广应用价值。

Study on the Enrichment and Purification Technology of Saponins from Mullugo pentaphylla L. with Macroporous Resin

Objective] The purpose was to research the technology of enriching and purifying the saponins from Mullugo pentaphylla L.with macroporous resins.Method] The method of static adsorption were used to confirm the suitable type of resin through selecting 4 resins with different types as AB-8,D101,HPD100 and HPD600 by using the technique of macroporous adsorption resin.The yield of total saponin was used as the index to investigate the conditions of its purification and enrichment by using the method of dynamic adsorption.Result] Among 4 types of resins,D101 macroporous resin was the most appropriate resin for the purification and enrichment of saponins from M.pentaphylla,with the elution rate for the saponins from M.pentaphylla of 80.9 % and the technology conditions were as follows: with 60% volume fraction of ethanol as the elution solvent,the solution concn.of 0.5 mg/ml,the elution velocity of 2 BV/h and the ratio of the resin to crude drug of 2∶1.Conclusion] The technology of enriching and purifying the saponins from M.pentaphylla with macroporous resins had advantages such as greater adsorption,higher elution rate and simple resin regeneration and had the popularizing and applying value in the enriching purification and production of saponins from M.pentaphylla.