| 护卵汤对多周期促排卵小鼠子宫内膜ER/PR蛋白表达的影响 |
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| 作者姓名： | 申奏秦旋 申可佳 熊桀 王肖 王宇航 吴华英 邓文 |
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| 作者单位： | 湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,郑州大学第一附属医院生殖中心, 河南 郑州 450052,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208 |
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| 基金项目： | 国家自然科学基金项目资助（81303003，81403427）；湖南省大学生研究性学习和创新性实验计划项目资助（181）。 |
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| 摘 要： | 目的 观察中药护卵汤对反复多周期促排卵小鼠子宫内膜容受性的干预作用，为中医药辅治体外受精-胚胎移植（IVF-ET）提供更多的实验依据。方法 建立反复多周期促排卵小鼠模型（模型组），部分给予中药护卵汤治疗（中药组），以及未使用药物的自然周期小鼠为正常对照（空白组）。比较各组在孕2 d、孕5 d两个时间段的子宫形态及雌激素受体（ER）和孕激素受体（PR）的蛋白表达。结果 子宫内膜光学形态：与空白组和中药组相比，孕5 d模型组小鼠子宫内膜发育明显不良。子宫内膜ER蛋白表达：与模型组相比，孕2 d和孕5 d中药组小鼠子宫内膜ER蛋白表达均增加，差异有统计学意义（P<0.05）；与空白组相比，孕5 d中药组小鼠子宫内膜ER蛋白表达增加，差异有统计学意义（P<0.05）。子宫内膜PR蛋白表达：孕2 d和孕5 d中药组小鼠子宫内膜PR蛋白表达均较模型组和空白组明显增加，差异有统计学意义（P<0.01）。结论 在西医多周期促排卵过程中辅以中药护卵汤，能促进子宫内膜生长发育，增加子宫内膜ER/PR蛋白表达，改善多周期促排卵小鼠的子宫内膜容受性。
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| 关 键 词： | 护卵汤 雌激素受体 孕激素受体 子宫内膜容受性 小鼠 |
| 收稿时间： | 2016/11/3 0:00:00 |
Effects of Huluan Decoction on Expression of ER and PR in Endometrium of Multi-Period Ovulation Induction Mice |
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| Authors: | SHEN Zouqinxuan SHEN Keji XIONG Jie WANG Xiao WANG Yuhang WU Huaying and DENG Wen |
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| Institution: | Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China and Hunan University of Chinese Medicine, Changsha, Hunan 410208, China |
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| Abstract: | Objective To observe the effects of Huluan decoction on expression of ER and PR in endometrium of multi-period ovulation induction mice, and to provide more related basis for the effectiveness of Huluantang assisting in vitro fertilization and embryo transfer (IVF-ET). Methods The multi-period ovulation induction mice models were built. The Chinese medicine group was administrated with Huluan decoction, the normal group was given no drugs. The morphology of endometrium and expression of ER and PR in endometrium were observed on the 2nd day and the 5th day of pregnant (P2 and P5). Results Compared with those in normal group and Chinese medicine group, the growth and development of the P5 endometrium in the model group was unhealthy. Compared with those in the model group, the expression of ER in the P2 and P5 endometriumin of Chinese medicine group increased significantly (P<0.05). Compared with those in the normal group, the expression of ER in the P5 endometrium of the Chinese medicine group increased significantly (P<0.05). The expression of PR in the P2 and P5 endometrium in the Chinese medicine group increased more obviously than those in model group and normal group (P<0.01). Conclusion The Chinese medicine of Huluan decoction could promote the growth and development of endometrium and increase the expression of ER and PR in endometrium, which may improve the endometrial receptivity in multi-period ovulation induction mice. |
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| Keywords: | Huluan decoction ER PR endometrial receptivity mice |
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