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蝴蝶兰组织培养中的褐化控制研究
引用本文:刘真华,葛红,郭绍霞,刘洪涛,曹灿景,周玉杰,李秋香.蝴蝶兰组织培养中的褐化控制研究[J].园艺学报,2005,32(4):732-737.
作者姓名:刘真华  葛红  郭绍霞  刘洪涛  曹灿景  周玉杰  李秋香
作者单位:中国农业科学院蔬菜花卉研究所, 北京100081; 莱阳农学院环境艺术系, 莱阳265200
摘    要: 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。

关 键 词:蝴蝶兰  组织培养  褐化  褐化控制
文章编号:0513-353X(2005)04-0732-03
收稿时间:2004-12-13
修稿时间:2004-12-132005-04-13

Studies of Antibrowning in the Tissue Culture of Phalaenopsis
Liu Zhenhua,Ge Hong,Guo Shaoxia,Liu Hongtao,Cao Canjing,Zhou Yujie,Li Qiuxiang.Studies of Antibrowning in the Tissue Culture of Phalaenopsis[J].Acta Horticulturae Sinica,2005,32(4):732-737.
Authors:Liu Zhenhua  Ge Hong  Guo Shaoxia  Liu Hongtao  Cao Canjing  Zhou Yujie  Li Qiuxiang
Institution:Institute of Vegetables and Flowers, Chinese Academ y of Agricultural Sciences, Beijing 100081, China; 2 The Landscape Architecture and Art Department, Laiyang Agricultural College, Laiyang 265200, China)
Abstract:In order to control the browning of tissue culture on Phalaenopsis,the contrast test of adsorbent: activated charcoal,PVP,and five antioxidants: citric acid,cysteine,glutathione,vitamin C,sodium thiosulfate in tissue culture of Phalaenopsis were studied.The results showed that activated charcoal can control browning and promote growth.But at the same time,it was adverse to the differentiation.Although the glutathione,which the effect of antibrowning was not as good as activated charcoal's,its resultant effect was the best one.
Keywords:Phalaenopsis spp    Tissue culture  Browning  Antibrowning
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