| 香石竹GA20-oxidase基因的克隆及RNA干扰载体的构建 |
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| 引用本文： | 金亮,孙振元,刘芸,李天红.香石竹GA20-oxidase基因的克隆及RNA干扰载体的构建[J].农业生物技术学报,2009,17(1):107-113. |
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| 作者姓名： | 金亮 孙振元 刘芸 李天红 |
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| 作者单位： | 1. 中国农业大学农学与生物技术学院果树系/北京市果树逆境生理与分子生物学重点开放实验室,北京,100193
2. 中国林业科学院花卉中心,北京,100093 |
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| 基金项目： | 教育部新世纪优秀人才支持计划，国家支撑计划，北京市教育委员会都市农业学科群建设项目 |
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| 摘 要： | 根据已发表的菠菜、烟草等植物GA 20-oxidase基因序列在保守区设计简并引物，通过RT-PCR和RACE的方法克隆了Marster香石竹（Dianthus caryophyllus L. cv. Marster）GA 20-oxidase基因的全长cDNA（1 179 bp），命名为Dc20ox。同源性分析表明该基因与其它作物上发表的GA 20-oxidase基因的氨基酸序列同源性为66%~75%。在此基础上选用同源性相对较高的400 bp DNA片段，构建了RNA干扰（RNAi）载体pART400。
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| 关 键 词： | 香石竹 GA 20-oxidase基因 RNAi |
| 收稿时间： | 2008-4-2 |
| 修稿时间： | 2008-4-25 |
Cloning of Carnation GA20-oxidase Gene and Construction of Plant RNAi Vector |
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| JIN Liang,SUN Zhen-yuan,LIU Yun,LI Tian-hong.Cloning of Carnation GA20-oxidase Gene and Construction of Plant RNAi Vector[J].Journal of Agricultural Biotechnology,2009,17(1):107-113. |
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| Authors: | JIN Liang SUN Zhen-yuan LIU Yun LI Tian-hong |
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| Abstract: | A pair of degenerate primers were designed based on the conserved domain of the GA 20-oxidase reported in other plants. A full-length cDNA (1 179 bp) of carnation (Dianthus caryophyllus L. cv. Marster) GA20-oxidase named Dc20ox was cloned by RT-PCR and RACE methods. The deduced amino acids of this sequence shared a high homology. Blast showed that the amino acids sequence presented a high homology (66%~75%) with the GA20-oxidase in other plants. A RNAi vector (pART400) was constructed by a high homology 400 bp fragment. |
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| Keywords: | RNAi |
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