| 梅与其近缘种亲缘关系的AFLP分析 |
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| 引用本文： | 明军,张启翔. 梅与其近缘种亲缘关系的AFLP分析[J]. 北京林业大学学报, 2012, 0(Z1): 88-91 |
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| 作者姓名： | 明军 张启翔 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所;北京林业大学园林学院 |
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| 基金项目： | 国家社会公益研究项目(2005DIB022);湖北省自然科学基金项目(2004ABA142,2001ABB118);林木花卉遗传育种教育部重点实验室开放基金(200605-12) |
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| 摘 要： | 以包括野梅在内的15个梅品种、1个野生种和其近缘种杏、山杏、桃、山桃、李和‘垂枝’毛樱桃、‘紫叶’李为试材,运用AFLP标记,采用7对引物组合(E-ACTM-CAT、E-GAM-CTC、E-ACTM-CCA、E-ACTM-CAC、E-ACTCTC、E-ACTCAG、E-ACCM-CAG)选择性扩增得到287个多态性条带的6888条带数据,使用NTSYSpc2.1t软件,采用Nei’s(72)遗传距离,SAHNClustering进行不加权成对算术平均法聚类分析,得到了梅品种与近缘种的亲缘关系与形态学、育种记录等相关记录相符的结论,并支持梅花二元分类系统的相关分类。
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| 关 键 词： | 梅 近缘种 亲缘关系 AFLP |
Genetic relationships between Prunus mume Sieb.et Zucc.and related species using AFLP markers |
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| MING Jun,;ZHANG Qi-xiang. Genetic relationships between Prunus mume Sieb.et Zucc.and related species using AFLP markers[J]. Journal of Beijing Forestry University, 2012, 0(Z1): 88-91 |
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| Authors: | MING Jun, ZHANG Qi-xiang |
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| Affiliation: | 1 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,100081,P.R.China;2 College of Landscape Architecture,Beijing Forestry University,100083,P.R.China |
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| Abstract: | The silver-stained AFLP analysis was employed to analyze the DNA fingerprints of 15 cultivars,a wild species and seven closely related species of mei (Prunus mume).A total of 287 loci and 6 888 bandings were capable of producing sharp fingerprint patterns with the primer combinations of E-ACT M-CAT,E-GA M-CTC,E-ACT M-CCA,E-ACT M-CAC,E-ACT CTC,E-ACT CAG and E-ACC M-CAG.NTSYSpc2.1t was used to carry out cluster analysis,adopting SAHN clustering of UPGMA and Nei’s(72) genetic distance.The relationships among the species were discussed.The result indicated that the genetic relationships between cultivars of P.mume and closely related species conformed to morphological classification and breeding records,supporting the viewpoint of "dual classification system for mei flowers". |
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| Keywords: | Prunus mume Sieb.et Zucc. closely related species genetic relationships amplified fragment length polymorphism (AFLP) |
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