|
|||||
|
|
| C型口蹄疫病毒单克隆抗体的制备与鉴定 | |
| 引用本文： | 杨沁,林彤,常惠芸,薛慧文. C型口蹄疫病毒单克隆抗体的制备与鉴定[J]. 甘肃农业大学学报, 2013, 48(1): 1-5 |
| 作者姓名： | 杨沁 林彤 常惠芸 薛慧文 |
| 作者单位： | 1. 甘肃农业大学动物医学院,甘肃兰州730070;中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室,甘肃省生物检测工程技术研究中心,甘肃兰州730046 2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室,甘肃省生物检测工程技术研究中心,甘肃兰州730046 3. 甘肃农业大学动物医学院,甘肃兰州,730070 |
| 基金项目： | 国家转基因重大专项(2011ZX08011-004;2009ZX08007-008B;2009ZX08006-002B) |
| 摘 要： | 将灭活的C型FMDV背部皮下多点注射免疫BABL/c小鼠,取小鼠脾细胞和Sp2/0骨髓瘤细胞在PEG1 500作用下融合,用有限稀释法进行亚克隆.以C型FMDV重组VP1蛋白为抗原,间接ELISA法筛选单抗.以O、Asial、A型FMDV以及猪水疱病病毒为抗原,间接ELISA法检测单抗特异性.获得2株抗C型FMDV的单抗3B3和6D5,亚型鉴定为IgG1、IgG3;腹水效价分别为1∶25 600和1∶51 200;中和效价分别为1∶1 280和1∶640;2株单抗与O、Asial、A型FMDV以及猪水疱病病毒间无交叉反应,表明这2株单抗为高特异性抗C型FMDV单抗.研究结果可为C型FMD的诊断及预防提供基础材料,也可用于病因学及免疫学研究. |
| 关 键 词： | C型口蹄疫病毒 单克隆抗体 ELISA 特异性鉴定 |
Preparation and identification of monoclonal antibody against foot-and-mouth disease virus C serotype |
|
| YANG Qin , LIN Tong , CHANG Hui-yun , XUE Hui-wen. Preparation and identification of monoclonal antibody against foot-and-mouth disease virus C serotype[J]. Journal of Gansu Agricultural University, 2013, 48(1): 1-5 | |
| Authors: | YANG Qin LIN Tong CHANG Hui-yun XUE Hui-wen |
| Affiliation: | 1(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.State Key Laboratory of Veterinary Etiological Biology,National Foot and Mouth Disease Reference Laboratory,Engineering Research Center of Biological Detection of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
| Abstract: | BABL/c mouse was immunized with serotype C FMDV by subcutaneous multisite injection on its back.The hybridoma was prepared by the fusion of mouse spleen cell and Sp2/0 myeloma cell under the PEG1 500 treatment,and subcloned by limiting dilution.To screen monoclonal antibody,the indirect-ELISA was used with FMDV type C recombinant VP1 protein as an antigen.To detect the specificity of monoclonal antibody,the indirect-ELISA was used with the O,Asial,A serotypes of FMDV and SVDV as antigens.Two strains of monoclonal antibody(3B3,6D5) were obtained.The subtype of 3B3 was IgG1,while the subtype of 3B3 was IgG3.The titers of 3B3 and 6D5 in ascite were 1∶25 600 and 1∶51 200,respectively,while the neutralizing titers were 1∶1 280 and 1∶640,respectively.The two strains had no cross-reaction with the O,Asial,A serotypes of FMDV and SVDV.The two strains were identified as monoclonal antibodies against C serotype of FMDV with high specificity and could be used in diagnostic,prevention,and study of etiology. |
| Keywords: | foot-and-mouth disease virus(FMDV) C serotype monoclonal antibody(McAb) ELISA specificity identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |