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| 鸭坦布苏病毒E基因DNA疫苗构建及免疫原性的初步研究 | |
| 引用本文： | 徐大伟,李国新,李雪松,姬希文,李云章,李泽君.鸭坦布苏病毒E基因DNA疫苗构建及免疫原性的初步研究[J].中国预防兽医学报,2012,34(4):305-308. |
| 作者姓名： | 徐大伟 李国新 李雪松 姬希文 李云章 李泽君 |
| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特010018;中国农业科学院上海兽医研究所农业部寄生虫学重点开放实验室,上海200241 2. 中国农业科学院上海兽医研究所农业部寄生虫学重点开放实验室,上海,200241 3. 内蒙古农业大学兽医学院,内蒙古呼和浩特,010018 4. 中国农业科学院上海兽医研究所农业部寄生虫学重点开放实验室，上海200241;国家家禽工程技术研究中心，上海2002 |
| 基金项目： | 公益性行业(农业)科研专项经费项目(201003012);自然科学基金面上项目(31172332) |
| 摘 要： | 鸭坦布苏病毒(DTMUV)感染引起产蛋鸭产蛋急剧下降.目前,没有预防该病的疫苗.为研究DTMUV DNA疫苗的免疫效果,本研究将DTMUV的E基因插入pCAGGS载体,构建了重组质粒pCAGGS-E.将pCAGGS-E转染293T细胞后,采用间接免疫荧光(IFA)和western blot检测E蛋白的表达情况.结果显示,两种方法均检测到了E蛋白的特异性表达.将pCAGGS-E用脂质体包裹后通过尾静脉注射免疫BALB/c小鼠,采用间接ELISA检测抗体的产生情况.结果表明,随着免疫次数的增加及免疫时间的延长,免疫小鼠的抗体滴度逐渐升高.本研究证明,编码E基因的重组质粒DNA免疫小鼠后能够诱导有效的免疫应答,为DTMUV DNA疫苗的研究奠定了基础. |
| 关 键 词： | 鸭坦布苏病毒 DNA疫苗 E基因 |
Construction and immunogenicity of DNA vaccine encoding E gene of duck Tembusu virus |
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| XU Da-wei , LI Guo-xin , LI Xue-song , JI Xi-wen , LI Yun-zhang , LI Ze-jun.Construction and immunogenicity of DNA vaccine encoding E gene of duck Tembusu virus[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):305-308. | |
| Authors: | XU Da-wei LI Guo-xin LI Xue-song JI Xi-wen LI Yun-zhang LI Ze-jun |
| Institution: | 2,3(1.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Key Laboratory of Animal Parasitology of the Ministry of Agricultural Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China; 3.National Poultry Engineering and Technology Research Center,Shanghai 200241,China) |
| Abstract: | Duck Tembusu virus(DTMUV) infection leads to decrease dramatically in egg production of laying duck.However,no vaccine against DTMUV is available at present.To investigate the immunogenicity of DNA vaccine against DTMUV,the E gene of DTMUV was cloned into eukaryotic expression vector pCAGGS to construct pCAGGS-E and the expression of E protein was detected in pCAGGS-E transfected 293T cells by indirect immunofluorescence assay and western blot.Furthermore,the immunogenicity was evaluated in BALB/c mice with the liposome capsuled pCAGGS-E via intravenous injection.The specific antibodies were detected by indirect ELISA,which showed the antibody titers increased gradually after pCAGGS-E multiple inoculations.In conclusion,the pCAGGS-E was able to induced specific immune responses in mice. |
| Keywords: | duck Tembusu virus DNA vaccine E gene |
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