Immune protection in carp, Cyprinus carpio L., after immunization with Aeromonas hydrophila crude lipopolysaccharide |
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| Authors: | T BABA J IMAMURA K IZAWA K IKEDA |
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| Institution: | Department of Veterinary Microbiology, University of Osaka Prefecture, Sakai, Osaka, Japan;National Research Institute of Aquaculture, Tamaki, Mie, Japan |
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| Abstract: | Abstract. Vaccination with crude lipopolysaccharide (LPS) induced better protection against infection with Aeromonas hydrophila in carp than vaccination with formalin killed vaccine. Dipping fish in vaccine for 2 h at 25°C was more effective than intraperitoneal injection of the vaccine in procedural simplicity, lower stress loading and the degree of protection acquired. In carp immunized with crude LPS by the dip method, antibodies were not detected by bacteriai agglutination, passive haemagglutination and the agar diffusion tests. The results indicate that the protection against A. hydrophila infection in carp is not dependent on humoral immunity. |
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