Leptin Receptor mRNA in Bull Ejaculated Spermatozoa |
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| Authors: | G Nikbakht M Roostaei Ali Mehr A Baghbanzadeh P Tajik C Tamanini M Emam |
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| Affiliation: | 1. Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;2. Department of Animal Science, Faculty of Agriculture sciences, University of Guilan, Rasht, Iran;3. Section of Physiology, Department of Basic science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;4. Department of Clinical Science, Faculty of veterinary medicine, University of Tehran, Tehran, Iran;5. Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali (DIMORFIPA), Universita` di Bologna, Bologna, Italy |
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| Abstract: | Leptin is a potential satiety factor and plays an important role in both metabolism and reproduction; both leptin and its receptor (Ob‐R) have been detected in human spermatozoa, thus suggesting leptin involvement in male gamete physiology. This experiment was designed to investigate leptin receptor [the long isoform (Ob‐Rb)] mRNA in bull ejaculated spermatozoa by RT‐PCR and southern hybridization. Total RNA was isolated from ejaculated spermatozoa and purified by different methods. Although the concentrations of RNA determined by all methods (except SDS/Proteinase K, lowest amount of RNA recovery) were similar, ethidium bromide staining was only detectable in lanes containing the samples isolated by sodium dodecyl sulphate (SDS) and SDS/citric acid extraction which produced higher RNA concentration. Ob‐Rb mRNA was detected in all samples using southern hybridization after RT‐PCR; it was shown only in three of them by RT‐PCR. We may conclude that Ob‐Rb mRNA is present in bull spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated in humans and pigs. |
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