| 植物内生菌研究进展及其存在的问题 |
| |
| 引用本文： | 李艳冰, 林亮, 廖秋红, 等. 促进滇重楼皂苷类活性成分积累的内生真菌筛选[J]. 云南农业大学学报（自然科学）, 2019, 34(1): 132-137. DOI: 10.12101/j.issn.1004-390X(n).201708036 |
| |
| 作者姓名： | 李艳冰 林亮 廖秋红 杨生超 刘涛 |
| |
| 作者单位： | 1.云南农业大学 农学与生物技术学院，云南 昆明 650201;2.西南中药材种质创新与利用国家地方联合工程研究中心，云南 昆明 650201;3.中国科学院 昆明植物研究所，云南 昆明 650201 |
| |
| 基金项目： | 国家自然科学基金项目（81473310，31560085） |
| |
| 摘 要： | 
ttttt目的ttttt植物内生菌(Endophyte)是近年来深受重视的一种新型的微生物能源，具有固氮、促进植物生长、增强宿主植物抗逆性等作用，目前研究表明：内生菌可增强宿主植物合成活性物质的能力，甚至它们本身也能产生与宿主植物相同或相近的生物活性物质。ttttttttttttt方法ttttt以传统药用植物滇重楼(Pairs polyphylla var.yunnanensis)为研究对象，从滇重楼地下块茎中分离得到23株内生菌，对其进行分子鉴定，之后与滇重楼组培苗进行共生培养，一段时间后利用高效液相色谱法检测组培苗的皂苷含量。tttttttttttttttttt结果ttttt最终筛选出2株对滇重楼组培苗皂苷含量有促进作用的菌株。ttttttttttttt结论ttttt人参生柱隔孢和木霉菌能促进滇重楼皂苷类活性成分的积累。tttt
|
| 关 键 词： | 滇重楼 内生真菌 ITS序列 |
| 收稿时间： | 2017-08-25 |
| 修稿时间： | 2018-05-07 |
Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew |
| |
| Yanbing LI, Liang LIN, Qiuhong LIAO, et al. Screening of Endophytic Fungi for Promote the Accumulation of Active Components of Saponins from Pairs polyphylla var. yunnanensis[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 132-137. DOI: 10.12101/j.issn.1004-390X(n).201708036 |
| |
| Authors: | Yanbing LI Liang LIN Qiuhong LIAO Shengchao YANG Tao LIU |
| |
| Affiliation: | 1.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;2.National Local Joint Engineering Research Center on Gemplasm Utilization and Innovation of Chinese Medicinal Materials in Southwest China, Kunming 650201, China;3.Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China |
| |
| Abstract: |
tttttPurposetttttEndophyte have got much attention as a novel microbial energy source in recent years. Its functions include nitrogen fixation, plant growth promotion and stress resistance of host plants. It is thought that endophyte can produce the same or similarity substances as host plant. Furthermore, some endophytes can also help host plant synthesis more active ingredients.tttttttttttttMethodtttttIn this study, 23 strains of endophytic fungi were isolated from the tuberous tissue of Paris polyphylla var. yunnanensis, and then cultured with the tissue culture plant of P. polyphylla var.yunnanensis, after culturing for a certain time.ttttttttttttttttttResultstttttTwo strains were screened for their increase of saponin content in tissue culture seedlings.tttttttttttttConclusiontttttCylindrocarpon sp. and Trichoderma viride can promote the accumulation of active constituent of P. polyphylla var. yunnanensis.ttttttttt
|
| |
| Keywords: | Pairs polyphylla var. yunnanensis endophytic fungi ITS sequences |
|
| 点击此处可从《云南农业大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《云南农业大学学报(自然科学版)》下载全文 |
