木霉菌M2的鉴定及其对小白菜促生效果研究

以一株分离自健康、高产辣椒根际土壤的木霉菌（Trichoderma spp.）M2 为供试菌株，研究其对小白菜生长发育的影响并在形态学分类的基础上结合rDNA-ITS 序列分析的手段，对木霉菌M2 进行分类鉴定，以期为微生物肥料的开发提供优良菌种。将木霉菌M2 进行固体发酵制得孢子粉，研究其在温室条件下是否对小白菜的生长具有促生能力。结果表明：菌株M2 属于哈茨木霉（Trichoderma harzianum）；在温室盆栽试验中，土壤接种M2 孢子粉对小白菜具有不同程度的增产作用，且能够提高叶绿素含量、可食用叶片数，其中接种5.0×10⁹ cfu 的M2 孢子粉处理的鲜重、干重最高，比对照组分别增加30.26%、20.08%；接种5.0×10⁸ cfu 的M2孢子粉处理鲜重、干重较对照组分别增加18.33%、12.46%。这说明哈茨木霉M2 菌株对小白菜的增产效果明显，具有潜在的农业应用价值。

Identification of Trichoderma Strain M2 and Related Growth Promoting Effects on Brassica chinensis L.

The research took Trichoderma strain as tested strains which isolated from the rhizosphere of healthy and high yield pepper, M2 was classified and identified by combining morphological classification with molecular identification means(rDNA-ITS sequence analysis), whose effect on growth of Brassica chinensis L. was explored, in order to provide superior strains for bio-fertilizer development. After solid fermentation of Trichoderma M2, under the condition of the greenhouse, took some research on the growth promoting effect on Brassica chi nensis L. of Trichoderma M2. The results showed that the strain M2 was identified as Trichoderma harzianum strain. M2 had obvious effects on promoting growth, the SPAD and edible leaf number of Brassica chinensis L. Adding of 5.0×10⁹ cfu M2 had the best effect on increasing the biological yield. Compared with CK, fresh weight and dry weight was increased by 30.26%and 20.08%respectively. Followed by inocula tion of 5.0×10⁸ cfu M2, fresh weight and dry weight was increased by 18.33% and 12.46% respectively. Therefore, M2 showed evident promoting effect on Brassica chinensis L., and had potential application value.