Enzymatic saccharification of water-soluble portion after hot-compressed water treatment of Japanese beech with xylanase and <Emphasis Type="Italic">β</Emphasis>-xylosidase |
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Authors: | Toshiki Nakata Hisashi Miyafuji Shiro Saka |
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Institution: | (1) Department of Socio-Environmental Energy Science, Graduate School of Energy Science, Kyoto University, Kyoto 606-8501, Japan |
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Abstract: | Enzymatic saccharifications of the hydrolysate collected as the water-soluble (WS) portion after hot-compressed water (HCW)
treatment of Japanese beech with xylanase and β-xylosidase were performed to investigate the hydrolytic properties of these enzymes. The WS portions with different molecular
weight distributions and initial concentrations of xylooligosaccharides were prepared as substrates, one of which contained
mainly smaller xylooligosaccharides degree of polymerization (DP) = 2 or 3], while the other contained larger xylooligosaccharides
(DP ≥ 4). The highest xylose recovery was obtained from both of the WS portions treated with β-xylosidase rather than with xylanase. This suggests that β-xylosidase could have a higher activity than xylanase toward larger xylooligosaccharides (DP ≥ 4) as well as smaller xylooligosaccharides
(DP = 2 or 3) recovered from HCW treatment. As a result, the use of β-xylosidase was found to be effective for saccharifi cation of the WS portion, even with a short reaction time for the HCW
treatment. |
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Keywords: | Enzymatic saccharification Water-soluble portion Hot-compressed water treatment Xylanase β -Xylosidase |
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