青花菜小孢子胚植株再生及倍性研究

以庆农70和庆农80为试材对青花菜小孢子胚状体植株再生因素进行研究。结果表明:胚龄为30～35d且发育健壮的子叶形胚再生成苗率最高,超过75%,胚龄超过40d再生成苗率显著降低;琼脂浓度为1.2%时,小孢子胚再生成苗率最高,达60%～70%;大多小孢子胚不能直接成苗,二次分化频率较高;植株继代和复壮培养适宜培养基为MS+3%蔗糖+1.0%琼脂;小孢子再生植株一半左右为单倍体,二倍体率为30%～35%,存在少数四倍体和嵌合体。

Studies on Plant Regeneration and Ploidy Identification of Microspore Embryiods in Broccoli(Brassica oleracea L.var.italica Plenck)

Taking broccoli(Brassica oleracea L.var.italica Plenck)variety‘Qingnong 70’and‘Qingnong 80’as experimental materials,this paper studies on plant regeneration factors of microspore embryoids.The results showed that the embryoids age(30-35 days)was optimal to plant regeneration,and the regeneration frequency exceeded 75 %,while it decreased significantly when the embryoids age was over 40 days.The regeneration frequency reached the highest(up to 60 %-70 %)when agar in the medium was 1.2 %.Most of embryoids could not produce plantlets directly,subculture was needed,and this treatment helped to differentiate much more plantlets.The optimal media for subculture was MS+3 % sucrose+1.0 % agar.About half of the regenerated plants were haploid,30 %-35 % plants were double haploid,and the rest were a few tetraploid or chimeras.