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Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide |
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| Authors: | Y. KaburakiT. Fujimura K. KurataK. Masuda M. TodaH. Yasueda K. ChidaS. Kawarai M. Sakaguchi |
| Affiliation: | a Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan b Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan c Institute of Tokyo Environmental Allergy, 2-2-4 Yushima, Bunkyo-ku, Tokyo 113-0034, Japan d Animal Allergy Clinical Laboratories, 5-4-21 Nishihashimoto, Sagamihara, Kanagawa 229-1131, Japan e Experimental Allergology, Paul-Ehrlich-Institut, Paul-Ehrlich-Street 59, Langen 63225, Germany f Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, 18-1 Sakuradai, Sagamihara, Kanagawa 228-8522, Japan g Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan |
| Abstract: | We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1-CpG) induced IL-12 in the spleen cells of naïve mice. Cry j 1-CpG immunization of BALB/c mice suppressed anti-Cry j 1 IgE response and enhanced anti-Cry j 1 IgG2a to subsequent Cry j 1 and alum adjuvant injection. CD4+T cells isolated from the spleens in mice immunized with Cry j 1-CpG produced higher IFN-γ levels than did CD4+T cells obtained from mice as negative controls. Our results suggested that Cry j 1-CpG immunization can induce Cry j 1-specific Th1 immune responses, thereby inhibiting IgE response to the pollen allergen. |
| Keywords: | Allergens CpG Pollen IgE |
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