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Reactivity and prevalence of neutralizing antibodies against Japanese strains of bovine viral diarrhea virus subgenotypes |
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| Authors: | Fujiko MinamiMakoto Nagai Mika ItoTatsuhiko Matsuda Hikaru TakaiYoshiko Jinkawa Takeshi ShimanoMichiko Hayashi Yoshihisa SekiYoshihiro Sakoda Katsuaki SugiuraHiroomi Akashi |
| Institution: | a Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa 920-3101, Japan b Ishikawa Prefectual Livestock Research Center, Hodatsushimizu-cho, Ishikawa 929-1325, Japan c Ishikawa Hokubu Livestock Hygiene Service Center, Nanao, Ishikawa 929-2126, Japan d Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa-mura, Iwate 020-0173, Japan e Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan f Food and Agricultural Materials Inspection Center, 2-1 Shintoshin, Chuo-ku, Saitama, Saitama 330-9731, Japan g Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan |
| Abstract: | Bovine viral diarrhea virus (BVDV) field isolates show genetic and antigenic diversity. At least 14 subgenotypes of BVDV-1 and 4 of BVDV-2 have been identified in Artiodactyla worldwide. Of these, 6 subgenotypes of BVDV-1 and 1 of BVDV-2 have been isolated in Japan. Previously, we reported that each subgenotype virus expresses different antigenic characteristics. Here we investigated the reactivity of neutralizing antibodies against representative strains of Japanese BVDV subgenotypes using sera from 266 beef cattle to estimate the prevalence of this epidemic virus among cattle in Japan. Antibody titers at concentrations at least 4-fold higher than antibodies against other subgenotype viruses were considered subgenotype specific. Subgenotype-specific antibodies were detected from 117 (80.7%) of 145 sera samples (69.7% against BVDV-1a, 1.4% against BVDV-1b, 8.3% against BVDV-1c, and 1.4% against BVDV-2a). The results suggest that neutralization tests are useful in estimating currently epidemic subgenotypes of BVDV in the field. |
| Keywords: | Antigenic difference Bovine viral diarrhea virus Epidemic virus Neutralization test Subgenotype |
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