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百合远缘杂交的胚囊和胚胎发育及胚拯救
引用本文:刘凤栾,杨利平,尚爱芹,梁丽峰,刘金良. 百合远缘杂交的胚囊和胚胎发育及胚拯救[J]. 河北农业大学学报, 2009, 32(2)
作者姓名:刘凤栾  杨利平  尚爱芹  梁丽峰  刘金良
作者单位:河北农业大学,园艺学院,河北,保定,071001;河北农业大学,园艺学院,河北,保定,071001;河北农业大学,园艺学院,河北,保定,071001;河北农业大学,园艺学院,河北,保定,071001;河北农业大学,园艺学院,河北,保定,071001
基金项目:河北省自然科学基金,河北农业大学博士科研基金 
摘    要:采用石蜡切片和子房切片培养方法,对百合杂交组合‘多安娜’ב西伯利亚’和‘普瑞头’ב雪皇后’授粉前后胚囊(胚胎)发育以及胚拯救效果进行了研究。结果表明:‘多安娜’子房内成熟胚囊高峰期滞后于‘普瑞头’3 d左右,而开花后8、10 d时其保持成熟胚囊的比例高于后者。两杂交组合的双受精作用开始于授粉后10 d左右,较亚洲百合自交亲和组合晚,且均存在不同程度的受精后障碍。子房切片培养和胚珠培养仅使‘多安娜’ב西伯利亚’组合获得幼苗。授粉70 d后,两杂交组合都收获少量成熟种子,并经MS培养基培养30 d后萌发成苗。

关 键 词:百合  胚胎败育  胚拯救  不亲和  杂交障碍

Embryo sac and embryo development and embryo rescue in Lilium distant cross
LIU Feng-luan,YANG Li-ping,SHANG Ai-qin,LIANG Li-feng,LIU Jin-liang. Embryo sac and embryo development and embryo rescue in Lilium distant cross[J]. Journal of Agricultural University of Hebei, 2009, 32(2)
Authors:LIU Feng-luan  YANG Li-ping  SHANG Ai-qin  LIANG Li-feng  LIU Jin-liang
Affiliation:College of Horticulture;Agricultural University of Hebei;Baoding 071001;China
Abstract:The process of embryo sac and embryo development of 'Pollyanna'×'Siberia' and 'Prato'×'Snow Queen' were observed by paraffin sections,and the immature embryo was rescued by vary culture.The results showed that the peak of mature embryonic sac in 'Pollyanna' was 3 days late compared with 'Prato',but the proportion of mature embryo sac of 'Pollyanna' at 8 d and 10 d after anthesis was higher than that of 'Prato'.The double fertilization was observed after 10 d pollination in both combinations.There were higher fertilized obstacles at different degree in the two crosses,and the cross of 'Prato'×'Snow Queen' was more serious.Seedlings were obtained in 'Pollyanna'×'Siberia' by vary culture.
Keywords:lily  embryo abortion  embryo rescue  incompatible  crossing barrier  
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